Anti-Histone H3.3 Antibody FL490 Conjugate (N158/28)
Our Anti-Histone H3.3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N158/28. It detects human, mouse, and rat Histone H3.3, and is purified by Protein A chromatography. It is great for use in IHC.
SKU: 75-228-FL490
Product Details
Histone H3.3
H3 Histone Family Member 3B is encoded by the gene H3.3B. H3.3B is a member of the histone H3 family. H3.3B contains introns and its mRNA is polyadenylated. H3.3B is a variant histone H3, and is a replication-independent histone. H3.3B is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. H3.3B is expressed in 9 organs with the highest expression in the spleen. Diseases associated with H3.3B include Chondroblastoma, Clear Cell Chondrosarcoma, Bone Giant Cell Sarcoma, Malignant Giant Cell Tumor, and brain stem cancer.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
N158/28
IgG1
IHC
Mouse
H3f3b H3.3b
15 kDa
Synthetic peptide amino acids 83-93 (LRFQSAAIGAL) of rat Histone H3.3 (accession number P84245)
Human, Mouse, Rat
AB_2939832
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL490 Ex: 491 nm, Em: 515 nm
Does not cross-react with Histone H3.1
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from date of receipt
Shipped on ice packs
Histone H3.3
UniProt (Human): P84243
UniProt (Immunogen Species): P84245
UniProt (Immunogen Species): P84245