Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.
Anti-Histone H3.3 Antibody
H3 Histone Family Member 3B is encoded by the gene H3.3B. H3.3B is a member of the histone H3 family. H3.3B contains introns and its mRNA is polyadenylated. H3.3B is a variant histone H3, and is a replication-independent histone. H3.3B is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. H3.3B is expressed in 9 organs with the highest expression in the spleen. Diseases associated with H3.3B include Chondroblastoma, Clear Cell Chondrosarcoma, Bone Giant Cell Sarcoma, Malignant Giant Cell Tumor, and brain stem cancer.
Synthetic peptide amino acids 83-93 (LRFQSAAIGAL) of rat Histone H3.3 (also known as H3.3a/b and H3f3a/b, accession number P84245); Mouse: 100% identity (11/11 amino acids identical); Human: 100% identity (11/11 amino acids identical); Greater than 70% identity with Histone H3.2 and H3.1 (8/11 amino acids identical)
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with Histone H3.1
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.