Anti-Histone H3.3 Antibody (N158/28)

Name: Anti-Histone H3.3 Antibody
Brand: NeuroMab
SKU: 75-228
Price: 319.0 USD
Availability: InStock

Our Anti-Histone H3.3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N158/28. It detects human, mouse, and rat Histone H3.3, and is purified by Protein A chromatography. It is great for use in ELISA, IHC.

Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-228

Volume: 100 µL

Variant

Price:

$319

Quantity:

Product Details

Target

Histone H3.3

Target Description

H3 Histone Family Member 3B is encoded by the gene H3.3B. H3.3B is a member of the histone H3 family. H3.3B contains introns and its mRNA is polyadenylated. H3.3B is a variant histone H3, and is a replication-independent histone. H3.3B is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. H3.3B is expressed in 9 organs with the highest expression in the spleen. Diseases associated with H3.3B include Chondroblastoma, Clear Cell Chondrosarcoma, Bone Giant Cell Sarcoma, Malignant Giant Cell Tumor, and brain stem cancer.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N158/28

Isotype

IgG1

Applications

ELISA, IHC

Host Species

Mouse

Gene Name

H3f3b H3.3b

Molecular Weight

15 kDa

Antigen

Synthetic peptide amino acids 83-93 (LRFQSAAIGAL) of rat Histone H3.3 (accession number P84245)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315849

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Histone H3.1

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Histone H3.3

UniProt Details

UniProt (Human): P84243
UniProt (Immunogen Species): P84245

Satterlee JS, Beckel-Mitchener A, McAllister K, Procaccini DC, Rutter JL, Tyson FL, Chadwick LH. (2015), 'Community resources and technologies developed through the NIH Roadmap Epigenomics Program..' Methods in Molecular Biology. 10.1007/978-1-4939-1804-1_2.

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Anti-Histone H3.3 Antibody (N158/28)

SKU: 75-228

Product Details

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