Anti-GluN1/NR1 Glutamate Receptor Antibody (N308/48)

Name: Anti-GluN1/NR1 Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-272
Price: 394.0 USD
Availability: InStock

Our mouse GluN1/NR1 glutamate receptor monoclonal antibody from NeuroMab is produced in-house from clone N308/48. It detects human, mouse, and rat GluN1/NR1 glutamate receptor and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.$

Representative fluorescence images of Fbxo2 (green) and NR1 (red; cat. 75-272, 1:500) fluorescence intensity in PFC slices from WT or Tau mice treated with Smyd3 inhibitor BCI121 or vehicle. Image from publication CC-BY-4.0. PMID:36609445

Adult rat hippocampus immunohistochemistry.

$Western blot data showing NR1 protein levels at the synaptic fraction in PFC of WT or Tau mice treated with Smyd3 inhibitor BCI-121 or vehicle. Image from publication CC-BY-4.0. PMID:36609445$

Immunohistochemistry of FAM81A and GluN1 (Cat. 75-272) was performed on a section of adult mouse brain. Image from publication CC-BY-4.0. PMID:38452102

Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-272

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN1/NR1 glutamate receptor

Scientific Background

Glutamate receptor 1, NMDA 1, or GluN1/NR1 also known as subunit 1 of the N-methyl-D-aspartate (NMDA) receptor. It is a member of the glutamate receptor channel superfamily and is a heteromeric glutamate-gated calcium ion channel. GluN1 is essential for synaptic function in the brain. GluN1 is expressed throughout the brain in cerebellum, thalamus, olfactory bulb and cortex in the membrane at postsynaptic density. GluN1 plays a key role in synaptogenesis, synaptic plasticity, memory acquisition and learning. Mutations in the GluN1 gene are associated with intellectual disability and cortical visual impairment and movement disorders.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N308/48

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Grin1 Nmdar1

Molecular Weight

105 kDa

Immunogen

Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1 (accession number P35439) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11000180

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

GluN1; Glutamate receptor ionotropic; NR1; Glutamate [NMDA receptor subunit zeta-1; NMDA 1; N-methyl-D-aspartate receptor subunit NR1; N-methyl-D-aspartate glutamate receptor; NMD-R1; NMDA R1 receptor C1 cassette; neurotransmitter receptor

UniProt ID

P35439
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.

NCBI Gene ID

24408

Shipping

Shipped on ice packs

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Anti-GluN1/NR1 Glutamate Receptor Antibody (N308/48)

SKU: 75-272

Product Details

Bulk Order Anti-GluN1/NR1 Glutamate Receptor Antibody

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