Anti-GAD65/67 Antibody (L127/12)

Name: Anti-GAD65/67 Antibody
Brand: NeuroMab
SKU: 75-508
Price: 329.0 USD
Availability: InStock

Our Anti-GAD65/67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Western blotting of rat brain homogenate (10 ug/lane) with anti-GAD65/67 (L127/12) at 1:100 dilution and detected with anti-mouse HRP.

Confocal images of immunostained rat dendritic section of hippocampal neurons labeled with GAD65 (Cat 75-508, 1:100, blue), total NL2 (red), and synaptic NL2 (green). Neurons were overexpressing SNX27GFPWT (B), SNX27GFPH112A (C), or mock transfected (A, control). Image from publication CC-BY-4.0. PMID 31775031

Western blotting of rat brain homogenate (10 ug/lane) with anti-GAD65/67 (L127/12) at 1:100 dilution and detected with anti-mouse HRP.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-508

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GAD65/67

Target Description

There are two forms of the enzyme glutamic-acid decarboxylase (GAD), a 65-kDa form (GAD65) and a 67-kDa form (GAD67) which are the result of the expression of different genes. Further splicing of GAD 67 produces one or two smaller variants, including GAD25 and GAD44. GAD67 and GAD65 are expressed in adult brain and the pancreas. The truncated forms GAD25 and GAD44 are expressed during embryonic development. Glutamic acid decarboxylase enzyme functions to catalyze the production of gamma-aminobutyric acid from L-glutamic acid.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L127/12

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GAD1, GAD, GAD67

Molecular Weight

70 kDa

Antigen

Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2756510

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

ICC: 1:100

Conjugate

Unconjugated

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1

UniProt ID

Q99259

Product Specific References for Applications and Species

Immunocytochemistry: Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
31775031	1:100	Halff, E.F., et al. 2019. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Reports, 2599-2607.

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Anti-GAD65/67 Antibody (L127/12)

SKU: 75-508

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-GAD65/67 Antibody

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