Anti-GABA-B-R2 Antibody (N81/2)

Name: Anti-GABA-B-R2 Antibody
Brand: NeuroMab
SKU: 75-124
Price: 329.0 USD
Availability: InStock

Our Anti-GABA(B)R2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N81/2. It is KO validated, detects human and mouse GABA(B)R2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against adult rat brain membranes (RBM) and membranes from GABABR2 wild-type (WT) and knockout (KO) mice probed with N81/2 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts).

Immunoblot against crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N81/2 (left) or N52A/42 (right) TC supe.

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Species Reactivity

Human, Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-124

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(B)R2

Scientific Background

Gamma-aminobutyric acid type B receptor subunit 2 or GABA BR2 is encoded by the gene GABBR2. GABA BR2 is a subunit of the G-protein coupled receptor for the neurotransmitter GABA (gamma-aminobutyric acid). It will inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. GABA BR2 is highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus at the cell junction, synapse, the postsynaptic cell membrane and cytoplasmic vesicle membrane. It is weakly expressed in heart, testis and skeletal muscle. Diseases associated with GABBR2 include Neurodevelopmental Disorder With Poor Language And Loss Of Hand Skills, Rett syndrome- and Early Infantile Epileptic Encephalopathy 59.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N81/2

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GABBR2 GPR51 GPRC3B

Molecular Weight

105 kDa

Immunogen

Fusion protein amino acids 861-912 of rat GABABR2 (accession number NP_113990) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_10672845

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.51

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABABR1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Gamma-aminobutyric acid type B receptor subunit 2 (GABA-B receptor 2) (GABA-B-R2) (GABA-BR2) (GABABR2) (Gb2) (G-protein coupled receptor 51) (HG20)

UniProt ID

O75899

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse | Rat
PLA: Mouse
Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Mouse
PMID	Dilution	Publication
27666021	1:100	Wolfe, S.A., et al. 2016. FMRP regulates an ethanol-dependent shift in GABABR function and expression with rapid antidepressant properties. Nature Communications, 12867.

Immunocytochemistry: Rat
PMID	Dilution	Publication
34510281	1:500	Luquin, E., et al. 2022. Stereological estimations and neurochemical characterization of neurons expressing GABAA and GABAB receptors in the rat pedunculopontine and laterodorsal tegmental nuclei. Brain Structure and Function, 89-110.
25560757	not listed	Workman, E.R., et al. 2015. Rapid antidepressants stimulate the decoupling of GABA(B) receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Molecular Psychiatry, 298-310.
23829864	1:400	Murthy, R., et al. 2013. Post-transcriptional regulation of GABAB receptor and GIRK1 channels by Nogo receptor 1.. Molecular Brain, 30.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
22179605	not listed	Maity, B., et al. 2012. Regulator of G protein signaling 6 (RGS6) protein ensures coordination of motor movement by modulating GABAB receptor signaling.. The Journal of Biological Chemistry, 4972-4981.

Immunohistochemistry: Rat
PMID	Dilution	Publication
34510281	1:100	Luquin, E., et al. 2022. Stereological estimations and neurochemical characterization of neurons expressing GABAA and GABAB receptors in the rat pedunculopontine and laterodorsal tegmental nuclei. Brain Structure and Function, 89-110.

PLA: Mouse
PMID	Dilution	Publication
27666021	1:100	Wolfe, S.A., et al. 2016. FMRP regulates an ethanol-dependent shift in GABABR function and expression with rapid antidepressant properties. Nature Communications, 12867.

Western Blot: Human
PMID	Dilution	Publication
30642393	1:400	Pandya, M., et al. 2019. Sex- and age-related changes in GABA signaling components in the human cortex. Biology of Sex Differences, 5.
28213518	1:1000	Kang, J.Y., et al. 2017. Deficits in the activity of presynaptic γ-aminobutyric acid type B receptors contribute to altered neuronal excitability in fragile X syndrome. Journal of Biological Chemistry, 6621-6632.

Western Blot: Mouse
PMID	Dilution	Publication
30902970	not listed	Dinamarca, M.C., et al. 2019. Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing. Nature Communications, 1331.
28213518	1:1000	Kang, J.Y., et al. 2017. Deficits in the activity of presynaptic γ-aminobutyric acid type B receptors contribute to altered neuronal excitability in fragile X syndrome. Journal of Biological Chemistry, 6621-6632.
27552836	not listed	Huo, Q., et al. 2017. Prefrontal Cortical GABAergic Dysfunction Contributes to Aberrant UP-State Duration in APP Knockout Mice. Cerebral Cortex, 4060-4072.
27666021	1:800	Wolfe, S.A., et al. 2016. FMRP regulates an ethanol-dependent shift in GABABR function and expression with rapid antidepressant properties. Nature Communications, 12867.
22815516	1:2000	Nguyen, M.V., et al. 2013. MeCP2 is critical for maintaining mature neuronal networks and global brain anatomy during late stages of postnatal brain development and in the mature adult brain.. Journal of Neuroscience, 10021-10034.
22179605	not listed	Maity, B., et al. 2012. Regulator of G protein signaling 6 (RGS6) protein ensures coordination of motor movement by modulating GABAB receptor signaling.. The Journal of Biological Chemistry, 4972-4981.
21636656	1:750	Pacey, L.K., et al. 2011. Subchronic administration and combination metabotropic glutamate and GABAB receptor drug therapy in fragile X syndrome.. Journal of Pharmacology and Experimental Therapeutics, 897-905.

Western Blot: Rat
PMID	Dilution	Publication
25560757	not listed	Workman, E.R., et al. 2015. Rapid antidepressants stimulate the decoupling of GABA(B) receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. Molecular Psychiatry, 298-310.
23829864	1:400	Murthy, R., et al. 2013. Post-transcriptional regulation of GABAB receptor and GIRK1 channels by Nogo receptor 1.. Molecular Brain, 30.
20926668	1:2000	Nassirpour, R., et al. 2010. Morphine- and CaMKII-dependent enhancement of GIRK channel signaling in hippocampal neurons.. Journal of Neuroscience, 13419-13430.

Additional Publications: Unspecified
PMID	Publication
29107844	Zhang, J., et al. 2017. The mechanisms underlying olfactory deficits in apolipoprotein E-deficient mice: focus on olfactory epithelium and olfactory bulb. Neurobiology Aging, 20-33.
27342122	Zhang, J., et al. 2016. Altered striatal rhythmic activity in cylindromatosis knock-out mice due to enhanced GABAergic inhibition. Neuropharmacology, 260-267.
21762014	Lodewyks, C., et al. 2011. GABA-B receptor activation inhibits the in vitro migration of malignant hepatocytes.. Can The Journal of Physiology Pharmacol., 393-400.
21336828	Broussard, D.M., et al. 2011. Motor learning in the VOR: the cerebellar component.. Experimental Brain Research, 451-463.
21215802	Pacey, L.K., et al. 2011. Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse.. Molecular and Cellular Neuroscience, 563-572.

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Anti-GABA-B-R2 Antibody (N81/2)

SKU: 75-124

Product Details

Product Specific References for Applications and Species

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