Anti-CSPG4/NG2 Recombinant Chicken Chimeric mAb (6203-34)

Our chicken Anti-CSPG4/NG2 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone 6203-34. It detects human, mouse, and rat CSPG4/NG2 and is purified by affinity chromatography. It works in ELISA, ICC, IHC, WB.

Immunohistochemistry for detection of CSPG4/NG2 in adult rat hippocampus using Aves Labs’ anti-CSPG4/NG2 recombinant chicken monoclonal antibody [6203-34] (Cat No. 78-523). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-CSPG4/NG2 antibody (Cat No. 78-523) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Western blot detection of CSPG4/NG2 using Aves Labs’ anti-CSPG4/NG2 recombinant chicken monoclonal antibody [6203-34] (Cat No. 78-523). 4-12% Bis-Tris Gel was loaded with rat whole brain homogenate. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Primary antibody was incubated with sample at a concentration of 10 ug/ml (lane 1) and 2 ug/ml (lane 2) and then detected via chemiluminescence (7 min) using a Goat Anti-Chicken H+L, HRP conjugated secondary antibody. Anti-CSPG4/NG2 antibody recognizes endogenous NG2 in the rat whole brain homogenate at the expected molecular weight of ~250 kDa.
Immunohistochemistry for detection of CSPG4/NG2 in adult rat cerebellum using Aves Labs’ anti-CSPG4/NG2 recombinant chicken monoclonal antibody [6203-34] (Cat No. 78-523). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-CSPG4/NG2 antibody (Cat No. 78-523) was incubated with the sample at a dilution of 1:100 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of CSPG4/NG2 in adult rat hippocampus using Aves Labs’ anti-CSPG4/NG2 recombinant chicken monoclonal antibody [6203-34] (Cat No. 78-523). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-CSPG4/NG2 antibody (Cat No. 78-523) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of CSPG4/NG2 in adult rat hippocampus using Aves Labs’ anti-CSPG4/NG2 recombinant chicken monoclonal antibody [6203-34] (Cat No. 78-523). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-CSPG4/NG2 antibody (Cat No. 78-523) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
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Human, Mouse, Rat
ELISA, ICC, IHC, WB
Chicken
490
550
594
650
Carrier-Free

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SKU: 78-523

Volume: 100 µL
Price:
$481.00
Ships: 1-2 business days

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