Our Anti-Copper ATPase 2 (Wilson's disease protein) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L62/29. It detects human, mouse, and rat Copper ATPase 2 (Wilson's disease protein), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunoblot versus rat brain membranes (RBM), rat liver membranes (RLM) and lysates of COS-1 cells transfected with human Copper- transporting ATPase 2 (COS+) or empty vector (COS-) and probed with L62/29 tissue culture supernatant.
Copper-transporting ATPase 2 or Copper-transporting ATPase Beta is encoded by the gene ATP7B and belongs to the cation transport ATPase (P-type) family. Copper-transporting ATPase 2 is a transmembrane enzyme that functions to provide copper to copper requiring proteins in the golgi network and is also to remove excess copper across plasma membranes out of the cell Copper-transporting ATPase 2 is expressed in liver and in lower leves in brain, heart, kidney, placenta and lung. In liver, it is involved in removing copper from hepatocytes into the bile for removal. Diseases associated with this gene include Wilson Disease and Menkes Disease.
Purified by Protein A chromatography
ICC, IHC, IP, WB
ATP7B PWD WC1 WND
160 kDa in rat brain membrane preparations
Synthetic peptide amino acids 3-21 (cytoplasmic N- terminus) of human Copper-transporting ATPase 2 (accession number P35670)
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.69
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.