Anti-Chapsyn-110 MAGUK Antibody (N18/28)

Name: Anti-Chapsyn-110 MAGUK Antibody
Brand: NeuroMab
SKU: 75-284
Price: 337.0 USD
Availability: InStock

Our mouse Chapsyn-110 MAGUK monoclonal antibody from NeuroMab is produced in-house from clone N18/28. It detects chicken, human, mouse, and rat Chapsyn-110 MAGUK and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot against crude membranes from whole adult mouse brain (MBM) or rat brain (RBM) and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N18/28 (left) or N52A/42 (right) TC supe.

Immunofluorescence staining of 14 DIV cultured rat hippocampal neurons.

Adult rat brain hippocampus immunohistochemistry.

Adult rat Cerebellum immunohistochemistry.

Immunoblot against crude membrane preparations from adult rat brain (RBM) or hippocampi from adult wild-type (WT) or PSD- 93/Chapsyn-110 knockout (KO) mice probed with N18/28 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University).

Immunoblot against crude membranes from whole adult mouse brain (MBM) or rat brain (RBM) and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N18/28 (left) or N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-284

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Chapsyn-110 MAGUK

Scientific Background

PSD-93/Chapsyn-110 (also known as Channel-associated protein of synapse-110, Postsynaptic density protein PSD-93, Disks large homolog 2 and Dlg2) is a member of the membrane-associated guanylate kinase (MAGUK) family. The protein contains a guanylate kinase-like domain, 3 PDZ domains and 1 SH3 domain. Chapsyn110 binds to NMDA receptor subunits and Shaker K+ channel subunits to aid in forming a multiprotein scaffold which clusters receptors, ion channels, cytoskeletal protiens and other cell signaling proteins. In this manner, it plays a key role in postsynaptic targeting. It is found in brain in neuronal cells and located at the cell synapse, postsynaptic cell membrane and postsynaptic density. Chapsyn110 has been implicated in chronic pain, bilateral retinoblastoma and schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N18/28

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Dlg2 Dlgh2

Molecular Weight

110 kDa

Immunogen

Fusion protein amino acids 1-852 (full-length) of rat Chapsyn-110 (accession number Q63622) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_11001825

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

Does not cross-react with SAP97, SAP102 or PSD-95

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93)

UniProt ID

Q63622

Shipping

Shipped on ice packs

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Anti-Chapsyn-110 MAGUK Antibody (N18/28)

SKU: 75-284

Product Details

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