Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb (N500/4)

Our chicken Anti-Cav1.2 Ca2+ Channel recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N500/4. It detects human, mouse, and rat Cav1.2 Ca2+ Channel and is purified by affinity chromatography. It works in ELISA, ICC, IHC, WB.

Immunohistochemistry for detection of Cav1.2 calcium channel in adult rat hippocampus using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Cav1.2 antibody (Cat No. 78-535) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Western blot detection of Cav1.2 Ca2+ channel using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). 4-12% Bis-Tris Gel was loaded with rat brain membrane fraction. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-Cav1.2 antibody was incubated with sample at a concentration of 2 ug/ml and then detected via chemiluminescence (6 min) using a Goat Anti-Chicken H+L, HRP conjugated secondary antibody. Anti-Cav1.2 recognizes endogenous Cav1.2 calcium channel in the rat brain membrane fraction at the expected molecular weight of ~240 kDa.
Immunohistochemistry for detection of Cav1.2 calcium channel in adult rat cerebellum using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Cav1.2 antibody (Cat No. 78-535) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of Cav1.2 calcium channel in adult rat cerebellum using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Cav1.2 antibody (Cat No. 78-535) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of Cav1.2 calcium channel in adult rat hippocampus using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Cav1.2 antibody (Cat No. 78-535) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of Cav1.2 calcium channel in adult rat hippocampus using Aves Labs’ anti-Cav1.2 recombinant chicken monoclonal antibody [N500/4] (Cat No. 78-535). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Cav1.2 antibody (Cat No. 78-535) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
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Human, Mouse, Rat
ELISA, ICC, IHC, WB
Chicken
490
550
594
650
Carrier-Free

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SKU: 78-535

Volume: 100 µL
Price:
$481.00
Ships: 1-2 business days

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