Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb FL490 Conjugate (N500/4)

Name: Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb FL490 Conjugate
Brand: Aves Labs
SKU: 78-535-FL490
Price: 562.0 USD
Availability: InStock

Our chicken Anti-Cav1.2 Ca2+ Channel FL490 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N500/4. It detects human, mouse, and rat Cav1.2 Ca2+ Channel and is purified by affinity chromatography. It works in ICC, IHC.

Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb FL490 Conjugate

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

Conjugates and Formulations

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-535-FL490

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Cav1.2 Ca2+ Channel

Scientific Background

Cav1.2 is the pore-forming subunit of the L-type voltage-gated calcium channel (VGCC). The necessity of calcium for heart contraction was identified in the 1880s, it wasn't until the late 1980s that the CACNA1C gene encoding Cav1.2 was successfully cloned and characterized. Cav1.2 is a "molecular transducer" that converts electrical signals into chemical actions. It is most prominent in cardiac and smooth muscle, as well as in neurons. When the cell membrane depolarizes, Cav1.2 undergoes a conformational change to allow Ca2+ ions to flow into the cytoplasm. In the heart, this influx is the first step for excitation-contraction coupling, as it triggers the RyR2 receptors to release the bulk of the calcium needed for a heartbeat. In the brain, Cav1.2-mediated calcium entry is vital for Long-Term Potentiation (LTP) and gene expression. Dysfunction in Cav1.2 is clinically linked to Timothy Syndrome, a severe multisystem disorder characterized by cardiac arrhythmias and autism.

Format

Purified by affinity chromatography

Concentration

0.5 mg/mL

Clonality

Recombinant

Clone

N500/4

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

Cacna1c

Molecular Weight

240 kDa

Excitation / Emission

FL490 Ex: 491 nm, Em: 515 nm

Immunogen

Recombinant protein amino acids 785-900 (ID I-II cytoplasmic loop) of mouse Cav1.2 (also known as voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c, accession number Q01815) produced recombinantly in E. Coli.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N500/4 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N500/4 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:100-1:500
ICC: 1:100-1:500

Conjugate

490

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c

UniProt ID

Q01815

Shipping

Shipped on ice packs

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Anti-Cav1.2 Ca2+ Channel Recombinant Chicken Chimeric mAb FL490 Conjugate (N500/4)

SKU: 78-535-FL490

Product Details

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