Anti-c-FOS Recombinant Chicken Chimeric mAb (N486/32)

Name: Anti-c-FOS Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-506
Price: 470.0 USD
Availability: InStock

Our Anti-c-FOS chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N486/32. It detects human, mouse, and rat c-FOS, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Immunofluorescent staining of serum starved (24 h) and serum stimulated (2 h) HeLa cells using indicated dilutions of Aves Labs anti-c-Fos recombinant chicken monoclonal antibody (Cat. 78-506) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-c-Fos specifically stains the nuclei of serum stimulated HeLa cells.

Western blotting of serum starved (24 h) and serum stimulated (2 h) HeLa cells using Aves Labs anti-c-Fos recombinant chicken monoclonal antibody (Cat. 78-506). The chicken mAb was detected with anti-chicken-HRP (Cat. 80-1000-HRP). Aves Labs Recombinant Chicken anti-c-Fos mAb recognizes endogenous c-Fos in the serum stimulated HeLa cells at ~65 kDa.

Immunohistochemistry staining of hippocampus region from adult rats treated with pilocarpine to induce seizures (top) and with saline (bottom) using anti-c-Fos antibody. The sections were stained with Antibodies Incorporated anti-c-Fos NeuroMab mouse monoclonal antibody (Cat. 75-506). The variable region of this antibody was used to generate recombinant chicken chimeric anti-c-Fos antibody (Cat. 78-506).

Immunofluorescent staining of serum starved (24 h) and serum stimulated (2 h) HeLa cells using indicated dilutions of Aves Labs anti-c-Fos recombinant chicken monoclonal antibody (Cat. 78-506) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-c-Fos specifically stains the nuclei of serum stimulated HeLa cells.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 78-506

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-5 business days

Product Details

Target

c-FOS

Scientific Background

C-fos belongs the Fos family. Other members include FosB, Fra-1, Fra-2. c-fos binds to JUN/AP1 transcription factor. C-Fos is expressed in placental tissue, fetal liver, developing bone, developing central nervous system, and adult bone marrow. It is expressed in the nucleus and in the endoplasmic reticulum. c-Fos has a role in signal transduction, cell proliferation, differentiation, and apoptosis. C-Fos is overexpressed in many cancers.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N486/32

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

FOS, G0S7

Molecular Weight

70 kDa

Immunogen

Fusion protein amino acids 1-377 (full-length) of human FOS (accession number P01100) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N486/32 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N486/32 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

UniProt ID

P01100

Shipping

Shipped on ice packs

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Anti-c-FOS Recombinant Chicken Chimeric mAb (N486/32)

SKU: 78-506

Product Details

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