Anti-BAF53a Antibody FL650 Conjugate (N336B/83)

Name: Anti-BAF53a Antibody FL650 Conjugate
Brand: NeuroMab
SKU: 75-330-FL650
Price: 486.0 USD
Availability: InStock

Our mouse BAF53a FL650 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N336B/83. It detects human, mouse, and rat BAF53a and is purified by Protein A chromatography. Works in IHC, ICC.

Anti-BAF53a Antibody FL650 Conjugate

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

594

Biotin

Carrier-Free

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Data Sheet

SKU: 75-330-FL650

Volume: 200 µL

Variant

Price:

$486.00

Quantity:

Ships: 1-5 business days

Product Details

Target

BAF53a

Scientific Background

Actin-like protein 6A, or BAF Complex 53 KDa Subunit (BAF53a) is encoded by the gene ACTL6A and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex). BAF53a is thought to be involved in transcriptional activation and repression of chromatin remodeling. BAF53a is a subunit of the neural progenitors-specific chromatin remodeling complex (npBAF complex). BAF53a is expressed in many different cell types. Diseases associated with ACTL6A include Coffin-siris syndrome and Brachymorphism-Onychodysplasia-Dysphalangism Syndrome.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Monoclonal

Clone

N336B/83

Isotype

IgG2b

Applications

ICC, IHC

Host Species

Mouse

Gene Name

ACTL6A BAF53 BAF53A INO80K

Molecular Weight

53 kDa

Immunogen

Fusion protein amino acids 43-119 (MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHP, actin subdomain 2) of human BAF53a (accession number O96019) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2940147

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

Specificity

Does not cross-react with BAF53b

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

Arp4; Actin-like protein 6A; BAF53A; 53 kDa BRG1-associated factor A; INO80K; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF complex 53 kDa subunit; BAF53; INO80 complex subunit K; actin-related protein 4; hArpN beta; ACTL6

UniProt ID

O96019
UniProt Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

NCBI Gene ID

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Anti-BAF53a Antibody FL650 Conjugate (N336B/83)

SKU: 75-330-FL650

Product Details

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