Our Anti-BAF53a mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N336B/83. It detects human, mouse, and rat BAF53a, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells.
Actin-like protein 6A, or BAF Complex 53 KDa Subunit (BAF53a) is encoded by the gene ACTL6A and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex). BAF53a is thought to be involved in transcriptional activation and repression of chromatin remodeling. BAF53a is a subunit of the neural progenitors-specific chromatin remodeling complex (npBAF complex). BAF53a is expressed in many different cell types. Diseases associated with ACTL6A include Coffin-siris syndrome and Brachymorphism-Onychodysplasia-Dysphalangism Syndrome.
Purified by Protein A chromatography
ACTL6A BAF53 BAF53A INO80K
Fusion protein amino acids 43-119 (MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHP, actin subdomain 2) of human BAF53a (accession number O96019) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Does not cross-react with BAF53b
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from date of receipt
Shipped on ice packs
Actin-like protein 6A (53 kDa BRG1-associated factor A) (Actin-related protein Baf53a) (ArpNbeta) (BRG1-associated factor 53A) (BAF53A) (INO80 complex subunit K)