Anti-Arl13b Recombinant Guinea Pig Chimeric mAb (N295B/66)
Our guinea pig Anti-Arl13b recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N295B/66. It detects human, mouse, non-human primate, rat, and zebrafish Arl13b and is purified by affinity chromatography. KO validated. Works in WB, IHC, ICC, ELISA.
![Immunohistochemistry for detection of Arl13b in adult rat brain lateral ventricle region using anti-Arl13b Recombinant Chimeric Guinea Pig Monoclonal antibody [N295B/66] (Cat No. 74-287) and co-stained with anti-GFAP Recombinant Chicken Monoclonal antibody [N206A/8] (Cat No. 78-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat. No AR-6591). The anti-Arl13b guinea pig antibody (Cat No. 74-240, 1:100) and anti-GFAP chicken antibody (Cat No. 78-240, 1:500) were incubated with the sample at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 (green) and goat anti-chicken IgY CF594 (red) secondary antibodies. DAPI nuclear stain (blue) shows cell nuclei. Anti-Arl13b specifically stains the ciliated cells surrounding the lateral ventricle.](http://www.antibodiesinc.com/cdn/shop/files/74-287-ihc-1_120x120.jpg?v=1778860436)
![Immunohistochemistry for detection of Arl13b in adult rat brain lateral ventricle region using anti-Arl13b Recombinant Chimeric Guinea Pig Monoclonal antibody [N295B/66] (Cat No. 74-287) and co-stained with anti-GFAP Recombinant Chicken Monoclonal antibody [N206A/8] (Cat No. 78-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-Arl13b guinea pig antibody (Cat No. 74-240, 1:100) and anti-GFAP chicken antibody (Cat No. 78-240, 1:500) were incubated with the sample at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 (green) and goat anti-chicken IgY CF594 (red) secondary antibodies. DAPI nuclear stain (blue) shows cell nuclei. Anti-Arl13b specifically stains the ciliated cells surrounding the lateral ventricle.](http://www.antibodiesinc.com/cdn/shop/files/74-287-ihc-2_120x120.jpg?v=1778860436)
![Immunohistochemistry for detection of Arl13b in adult rat brain lateral ventricle region using anti-Arl13b Recombinant Chimeric Guinea Pig Monoclonal antibody [N295B/66] (Cat No. 74-287) and co-stained with anti-GFAP Recombinant Chicken Monoclonal antibody [N206A/8] (Cat No. 78-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat. No AR-6591). The anti-Arl13b guinea pig antibody (Cat No. 74-240, 1:100) and anti-GFAP chicken antibody (Cat No. 78-240, 1:500) were incubated with the sample at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 (green) and goat anti-chicken IgY CF594 (red) secondary antibodies. DAPI nuclear stain (blue) shows cell nuclei. Anti-Arl13b specifically stains the ciliated cells surrounding the lateral ventricle.](http://www.antibodiesinc.com/cdn/shop/files/74-287-ihc-1_1600x.jpg?v=1778860436)
Immunohistochemistry for detection of Arl13b in adult rat brain lateral ventricle region using anti-Arl13b Recombinant Chimeric Guinea Pig Monoclonal antibody [N295B/66] (Cat No. 74-287) and co-stained with anti-GFAP Recombinant Chicken Monoclonal antibody [N206A/8] (Cat No. 78-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat. No AR-6591). The anti-Arl13b guinea pig antibody (Cat No. 74-240, 1:100) and anti-GFAP chicken antibody (Cat No. 78-240, 1:500) were incubated with the sample at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 (green) and goat anti-chicken IgY CF594 (red) secondary antibodies. DAPI nuclear stain (blue) shows cell nuclei. Anti-Arl13b specifically stains the ciliated cells surrounding the lateral ventricle.
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