Anoctamin-5, Transmembrane Protein 16E or -ANO5/TMEM16E is encoded by the gene ANO5. ANO5 belongs to the anoctamin family of membrane proteins many of which are calcium-activated chloride channels. ANO5 does not exhibit this channel activity. ANO5 is expressed in brain, heart, kidney, lung, skeletal muscle as well as other tissues. Diseases associated with ANO5 include Gnathodiaphyseal Dysplasia and Miyoshi Muscular Dystrophy 3.
Fusion protein amino acids 1-299 (cytoplasmic N-terminus) of human ANO5 (also known as Anoctamin-5, Gnathodiaphyseal dysplasia 1 protein, Transmembrane protein 16E, GDD1, TMEM16E and RGD1564265, accession number Q75V66)
Rat: 80% identity (228/283 amino acids identical)
Mouse: 77% identity (226/292 amino acids identical)
45% identity with ANO6/TMEM16F
Does not cross-react with ANO6/TMEM16F
Anoctamin-5 (Gnathodiaphyseal dysplasia 1 protein) (Transmembrane protein 16E)
ANO5 GDD1 TMEM16E
TC Supernatant: AB_2491107
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
Citations and References
- Griffin DA1, Johnson RW1, Whitlock JM2, Pozsgai ER3, Heller KN1, Grose WE1, Arnold WD4, Sahenk Z5, Hartzell HC2, Rodino-Klapac LR6.. (2016), 'Defective membrane fusion and repair in Anoctamin5-deficient muscular dystrophy..' Hum Mol Genet. 10.1093/hmg/ddw063.
- Vihola A1, Luque H1, Savarese M1, Penttilä S2, Lindfors M2, Leturcq F3, Eymard B4, Tasca G5, Brais B6, Conte T6, Charton K7, Richard I7, Udd B1,2,8.. (2017), 'Diagnostic anoctamin-5 protein defect in patients with ANO5-mutated muscular dystrophy..' Neuropathol Appl Neurobiol.. 10.1111/nan.12410.