Products: Primary Antibodies

Anti-ADAM11 Antibody (N441/35)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-432	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-432	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Disintegrin and metalloproteinase domain-containing protein 11, Metalloproteinase-Like, Disintegrin-Like, Cysteine-Rich Protein, or ADAM11 is encoded by the gene ADAM11 and a member of the ADAM (a disintegrin and metalloproteinase) family. ADAM11 is a single-pass type I membrane protein without catalytic metalloprotease-like properties. It is thought to be a ligand for integrin in the brain. ADAM11 is also weakly detected in the heart, liver and testis.

Immunogen:

Fusion protein amino acids 243-441 (extracellular domain) of mouse ADAM11 (also known as Disintegrin and metalloproteinase domain-containing protein 11, Metalloproteinase-like disintegrin-like and cysteine-rich protein, MDC and rCG_32562, accession number Q9R1V4)
Rat: 100% identity (199/199 amino acids identical)
Human: 99% identity (198/199 amino acids identical)
60% identity with ADAM22

Cross Reactivity:

Does not cross-react with ADAM22

Protein Name(s):

Disintegrin and metalloproteinase domain-containing protein 11 (ADAM 11) (Metalloproteinase-like, disintegrin-like, and cysteine-rich protein) (MDC)

Gene Name(s):

Adam11 Mdc

Antibody Registry ID:

Purified: AB_2636850
TC Supernatant: AB_2531889

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.