Anti-Cofilin 1 (N-terminus) Antibody

Name: Anti-Cofilin 1 (N-terminus) Antibody
Brand: PhosphoSolutions
SKU: CP1131
Price: 348.0 USD
Availability: InStock

Our Cofilin 1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Cofilin 1 (N-terminus) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin 1 (Ser-3) peptide. The blots were probed with anti-Cofilin 1 (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin 1 (N-terminus) (lanes 4-6).

Immunocytochemical labeling in chick dorsal root ganglion neurons using anti-Cofilin (N-terminus; CP1131), anti-Cofilin (Ser-3; CP1151), anti-βIII-Tubulin (C-terminus; TP1691) and anti-β-Tubulin (TM1541) antibodies. (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).

Western blot of cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin 1 (Ser-3) peptide. The blots were probed with anti-Cofilin 1 (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin 1 (N-terminus) (lanes 4-6).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: CP1131

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Cofilin 1 (N-terminus)

Scientific Background

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

CFL1

Molecular Weight

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the N-terminus of human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 19 kDa* protein corresponding to the molecular mass of Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CFL; ADF; cofilin; cofilin-1; 18 kDa phosphoprotein; cofilin 1 (non-muscle; non-muscle isoform; epididymis secretory protein Li 15; p18

UniProt ID

P23528
UniProt Summary: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.

NCBI Gene ID

1072

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Anti-Cofilin 1 (N-terminus) Antibody

SKU: CP1131

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