Anti-COBRA1 (NELF-B) Antibody (F7E4)

Name: Anti-COBRA1 (NELF-B) Antibody
Brand: PhosphoSolutions
SKU: 319-COBRA
Price: 456.0 USD
Availability: InStock

Our Anti-COBRA1 (NELF-B) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat COBRA1 (NELF-B) and is Protein G purified. It is great for use in WB.

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 319-COBRA

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

COBRA1 (NELF-B)

Scientific Background

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Format

Protein G Purified

Clonality

Monoclonal

Clone

F7E4

Isotype

IgG1

Applications

ELISA, WB

Host Species

Mouse

Gene Name

COBRA1

Molecular Weight

62 kDa

Immunogen

Recombinant full length human COBRA1 purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492059

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 0.05% NaN3

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~62 kDa COBRA1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

A730008L03Rik; AB041607; AI663983; Cofactor of BRCA1; DKFZp586B0519; KIAA1182; Negative elongation factor B; Negative elongation factor protein B; Nelf b; NELF-B; NELFB; RGD1307832; RP13-122B23.3; RP23-132N23.4

UniProt ID

Q8WX92
UniProt Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401). May be able to induce chromatin unfolding (PubMed:11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity). {ECO:0000250|UniProtKB:Q8C4Y3, ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:11739404, ECO:0000269|PubMed:12612062}.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed:23884411). In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed:23884411). {ECO:0000269|PubMed:23884411}.

NCBI Gene ID

25920

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134–46. PMID: 15342491
Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun. 325 (2): 568–73.PMID: 15530430

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Anti-COBRA1 (NELF-B) Antibody (F7E4)

SKU: 319-COBRA

Product Details

General References

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