Primary Antibodies

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Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Calnexin (CANX) (Cat. CANX-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-Calnexin specifically stains the Endoplasmic Reticulum in HeLa cells. ​Western blotting of various cell lysates with Aves Labs chicken anti-Calnexin (CANX) antibody (0.05 µg/ml) and detected with anti-chicken HRP. Aves Labs Chicken anti-Calnexin recognizes endogenous Calnexin in all the cell lysates at ~90 kDa.
Aves Labs Anti-Calnexin Antibody
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Immunostaining of HeLa cells showing specific labeling of Ki-67 (Cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.

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