Primary Antibodies

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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/38 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/36 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with untagged GRK5, GRK6 and Kv2.1 plasmids and probed with N142/28 TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) and untagged Kv2.1 plasmids and probed with N129A/6 (left) and K89/41 (right) TC supes.
Serial dilutions of KLH-conjugated and BSA-conjugated peptides dotted onto membrane and probed with N132A/12 TC supe (left) and a mouse loading control (right).
Transfected cell immunofluorescence: COS cells expressing Myc-tagged human Haspin. Red = rabbit anti-Myc, Green = N128A/2, Blue = Hoechst nuclear stain.Transfected cell immunoblot: extracts of COS cells transfected with Myc- tagged human Haspin and untagged Kv2.1 plasmids and probed with N128A/2 (left) and K89/41 (right) tissue culture supernatant.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N123/19 TC supe (left) and a mouse loading control (right).
Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N123/48 TC supe (left) and a mouse loading control (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L, DNMT3A and DNMT3B plasmids and probed with N117/9 TC supe (left) and mouse anti-Flag (right).Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain.
Transfected cell immunoblot: extracts of COS cells transfected with GFP-tagged MPP8 and untagged Kv2.1 plasmids and probed with N119/44 (left panel) and K89/34 (right panel) TC supe.Transfected cell immunofluorescence: COS cells expressing GFP-tagged human MPP8. Red = N119/44, Green = GFP, Blue = Hoechst nuclear stain.
Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn2 KO Mfn1 KO mice and probed with N111/24 TC supe. Samples courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.
Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1, -2, -3, -4 and -4* plasmids and probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane (RBM) immunoblot.
Adult rat hippocampus immunohistochemistry.Neuronal immunoblot: lysates from neocortical neurons cultured under amino acid starvation and probed with rabbit polyclonal anti-SNAT1 (left) and N104/32 (right). Data courtesy of Jeffery Erickson (LSUHSC)..
Immunoblot on adult rat brain membrane (RBM) and samples from transfected COS cells.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus whole cell extracts from embryonic day 15 telencephalon from DLX1/2 wild-type (WT) and knockout (KO) mice. Data courtesy of Dr. Mario Hernan and Dr. John Rubenstein, UCSF.
Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex layer 5 with L117/1 (red), rabbit mAb PSD-95 (Cell Signaling #3450, green) and DAPI (blue), where L117/1 colocalizes with some PSD-95 puncta.  Image courtesy of Kristina Micheva (Stanford)..Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with GFP-tagged SK1, SK3 or SK4 or untagged SK2 or Kv2.1 plasmid and probed with K78/29 (left) and N86/8 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.

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