Primary Antibodies

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Adult rat brain membrane immunoblot.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right).
Adult rat brain immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged SynDIG3 or untagged Kv2.1 plasmid and probed with N160/21 (left) and K89/41 (right) TC supe.
Immunostaining of spinal cord samples from WT and Gamma-protocadherin KO mice with N159/5 TC supe. Data courtesy of Joshua Weiner, University of Iowa.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc- tagged Uncx and untagged Kv2.1 plasmids and probed with N155/9 (left) and 19E10 (right) TC supes.Immunofluorescence of embryonic day 8.5 mouse embryo whole mounts showing posterior staining of somites (arrows). Data courtesy of Rieko Ajima and Terry Yamaguchi, Cancer and Developmental Biology Laboratory, National Cancer Institute.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged JMJD2A and untagged Kv2.1 plasmids and probed with N154/32 (left) and K89/41 (right).
Adult rat hippocampus immunohistochemistry.Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice and probed with N153/5 TC supe (top) and Porin antibody (bottom). Data courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids and probed with N150/21 (left), N86/8 (middle) and K89/41 (right).
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).Transfected HEK293 cell immunofluorescence staining N15/39=green, DAPI=blue.
Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.
Cerebellar lysate immunoblot probed with N151/3 (3 mg/mL).Cultured rat hippocampal neuron surface (S, left) and total (T, right) protein immunofluorescence staining (green) with N151/3 (2 mg/mL).
Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].Adult rat hippocampus immunohistochemistry (high and low magnification).
Adult rat brain membrane immunoblot.Immunoblot against adult rat brain membrane (RBM) and extracts of COS cells transiently transfected with untagged GRK5, GRK6 or Kv2.1 plasmid and probed with N145/20 TC supe.
NeuroMab Anti-Pan-GRK
Sale price$329
Immunoblot versus brain samples from WT and Gamma- protocadherin KO mice. Data courtesy of Joshua Weiner, University of Iowa.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/17 (left) and N159/5 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma-protocadherin-B2 and -B1 and untagged Kv2.1 plasmids and probed with N148/30 (left) and N86/6 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/38 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/36 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with untagged GRK5, GRK6 and Kv2.1 plasmids and probed with N142/28 TC supe.

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