Anti-VSP/TPTE Antibody (N432/21)

Name: Anti-VSP/TPTE Antibody
Brand: NeuroMab
SKU: 75-485
Price: 337.0 USD
Availability: InStock

Our mouse VSP/TPTE monoclonal antibody from NeuroMab is produced in-house from clone N432/21. It detects chicken, Xenopus, zebrafish VSP/TPTE and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe.

II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390

D. Dr-Vsp immunostaining in IECs LREs (right) of a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp expression was restricted mainly to LREs, but not detectable in other IECs. Green, Dr-Vsp. Red, F-actin. Blue, DAPI. Image from publication, CC-BY-4.0, PMID: 36088390

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Chicken, Xenopus, Zebrafish

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-485

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VSP/TPTE

Scientific Background

TPTEs (transmembrane phosphatases with tensin homology) aka VSPs (voltage sensing phosphatases) catalyze the dephosphorylation of phosphatidylinositol phosphates (PIPs) that regulate diverse aspects of cell membrane physiology including cell division, growth and migration (Ratzan W. et al., 2019). TPTEs are also thought to be involved in signal transduction pathways of the endocrine or spermatogenetic function of the testis (Chen H. et al., 1999).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N432/21

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

TPTE

Molecular Weight

40-60 kDa

Immunogen

Fusion protein amino acids 153-335 of zebrafish TPTE (accession number Q4V9E4) produced recombinantly in E. Coli

Immunogen Species

Zebrafish

Species Reactivity

Chicken, Xenopus, Zebrafish

Antibody Registry ID

AB_2744560

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Transmembranephosphatase with tensin homology (voltage-sensing phosphoinositide phosphatase) ( VSP)

UniProt ID

Q4V9E4

Shipping

Shipped on ice packs

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Anti-VSP/TPTE Antibody (N432/21)

SKU: 75-485

Product Details

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