Anti-VGlut2 Antibody (N29/29)

Name: Anti-VGlut2 Antibody
Brand: NeuroMab
SKU: 75-067
Price: 337.0 USD
Availability: InStock

Our mouse VGlut2 monoclonal antibody from NeuroMab is produced in-house from clone N29/29. It detects chicken, electric ray, human, mouse and rat VGlut2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.

Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.

Immunofluorescence staining of adult rat cerebellum with N29/29 (green), N326D/2 (red) and Hoechst nuclear stain (blue).

Adult rat cerebellum and thalamus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Chicken, Electric Ray, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-067

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGlut2

Scientific Background

Vesicular glutamate transporter 2 (Vglut2) is encoded by the gene Slc17a6 and belongs to the vesicular glutamate transporter family. Vglut2 is expressed in the thalamus, midbrain, brainstem, and spinal cord at synaptic vesicles. It functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut2 has been implicate in include Gnathodiaphyseal Dysplasia and tendinosis.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N29/29

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc17a6 Dnpi Vglut2

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession number Q9JI12) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Electric Ray, Human, Mouse, Rat

Antibody Registry ID

AB_2239153

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:400
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with VGlut1 or VGlut3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular glutamate transporter 2 (VGluT2) (Differentiation-associated BNPI) (Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 6)

UniProt ID

Q9JI12

Shipping

Shipped on ice packs

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Anti-VGlut2 Antibody (N29/29)

SKU: 75-067

Product Details

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