Anti-VGlut1 Antibody (N28/9)

Our mouse VGlut1 monoclonal antibody from NeuroMab is produced in-house from clone N28/9. It detects fish, human, mouse, and rat VGlut1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, Flow, EM, ELISA.

Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228
HEK293T transfectants expressing SLITRK1 or its mutants were co-cultured with hippocampal neurons. Two days after co-culture, induced pre-synapses were quantified through VGAT or VGLUT1 immunostaining and GFP. Image from publication CC-BY-4.0. PMID:36085162
Immunoblot against extracts of COS cells transfected with untagged VGlut or Kv2.1 plasmid and probed with N28/9 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult rat hippocampal immunohistochemistry.
Adult rat whole brain immunohistochemistry.
Representative images demonstrating vGlut1 (red) and NeuN (green) immunofluorescence staining in CA1 and cortex (CTX) of the mouse brain. Image from publication CC-BY-4.0. PMID:36465182
Representative images demonstrating vGlut1 (red) and Synapsin (green) immunofluorescence staining in CA1, DG, CA3, and CTX of the mouse brain. Image from publication CC-BY-4.0. PMID:36465182
Top row, Neurexin immunostainings from WT, LRRTM1 KO, SynCAM 1 KO and DKO mice in PFC layer II/III in anterior cingulate, prelimbic, and infralimbic areas at P50. Bottom row, co-staining Neurexin (magenta) with vGlut1 (Cat. 75-066, 1:800, green). Single 1.0 μm confocal sections were acquired. Insets show enlarged areas. Image from publication CC-BY-4.0. PMID:36672216
Representative immunostaining of hippocampal neurons cultured from Nrxn1ΔS5 mice showing specific labelling of PSD95 (red), VGlut1 (Cat. 75-066; yellow), and MAP2 (blue). Image from publication CC-BY-4.0. PMID:37384525
Images of dorsal and ventral forebrain organoids immunostained for VGlut1 (Cat. 75-066, 1:250) and GAD67. Image from publication CC-BY-4.0. PMID:38390494
Representative confocal image of a section of dendrite from DIV20 cortical neuron expressing eGFP and colabeled for ZFP804A and VGluT1 (Cat. 75-066, 1:200, blue). Colocalisation could be observed and confirmed by conducting orthogonal view analysis with VGLUT1, whereby the 2D ICC could be viewed and superimposed in 3D by producing an X,Y, Z plane. Scale bar = 10um. Image from publication, CC-BY-4.0. PMID: 38279611.
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228
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Fish, Human, Mouse, Rat
ELISA, EM, Flow, ICC, IHC, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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SKU: 75-066

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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