Anti-VE-Cadherin (Tyr-685), Phosphospecific Antibody

Name: Anti-VE-Cadherin (Tyr-685), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP1981
Price: 348.0 USD
Availability: InStock

Our VE-Cadherin (Tyr-685) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VE-Cadherin (Tyr-685) and is antigen affinity purified. It is great for use in WB.

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

Phosphorylation of CDH5 Y658, CDH5 Y685 (Cat. CP1981) and VEGFR2 (Tyr1054/1059) in cultured human LECs after Yoda1 (0.3 µM) treatment for the indicated periods. Image from publication CC-BY-4.0. PMID: 38528202

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: CP1981

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VE-Cadherin (Tyr-685)

Scientific Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

CDH5

Molecular Weight

140

Immunogen

Phospho-VE-Cadherin (Tyr-685) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 685 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse VE-cadherin, but is not conserved in other cadherins.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr-685) peptide before affinity purification using phospho-VE-cadherin (Tyr-685) peptide. The purified antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-685

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Cadherin-5, vascular endothelial Cadherin, CD144

UniProt ID

P33151

Shipping

Blue Ice

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Anti-VE-Cadherin (Tyr-685), Phosphospecific Antibody

SKU: CP1981

Product Details

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