Anti-VE-Cadherin (C-terminal) Antibody

Name: Anti-VE-Cadherin (C-terminal) Antibody
Brand: PhosphoSolutions
SKU: CP2231
Price: 285.0 USD
Availability: InStock

2 Citations

Our VE-Cadherin (C-terminal) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat VE-Cadherin (C-terminal) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: CP2231

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VE-Cadherin (C-terminal)

Scientific Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CDH5

Molecular Weight

140

Immunogen

VE-Cadherin synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse, and has less than 50% homology with other cadherins.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells, and this reactivity can be specifically blocked using VE-cadherin peptide (CX2235).

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Cadherin-5, vascular endothelial Cadherin, CD144

UniProt ID

P33151

Shipping

Blue Ice

Product Specific References for Applications and Species

Adhesion Assay: Mouse
Immunocytochemistry: Human

Adhesion Assay: Mouse
PMID	Dilution	Publication
20201897	25ug	Bowers, S.L., et al. 2010. The dynamics of fibroblast-myocyte-capillary interactions in the heart. Annals of the New York Academy of Sciences, 143-152.

Immunocytochemistry: Human
PMID	Dilution	Publication
30281335	not listed	Kenne, E., et al. 2019. Neutrophils engage the kallikrein-kinin system to open up the endothelial barrier in acute inflammation. FASEB Journal, 2599-2609.

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Anti-VE-Cadherin (C-terminal) Antibody

SKU: CP2231

Product Details

Product Specific References for Applications and Species

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