Anti-Slc18a2/VMAT2 Antibody (N449/73)

Name: Anti-Slc18a2/VMAT2 Antibody
Brand: NeuroMab
SKU: 75-436
Price: 337.0 USD
Availability: InStock

Our mouse Slc18a2/VMAT2 monoclonal antibody from NeuroMab is produced in-house from clone N449/73. It detects human, mouse, and rat Slc18a2/VMAT2 and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

$Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.$

Immunoblot against extracts of COS cells transiently transfected with Myc/Flag-tagged VMAT1, VMAT2, VAChT or Kv2.1 plasmid probed with N449/73 (left) or rabbit anti-Flag (right).

Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-436

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Slc18a2/VMAT2

Scientific Background

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N449/73

Isotype

IgG2b

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

Slc18a2

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 42-132 (first lumenal domain) of mouse Slc18a2 (accession number Q66L40) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2686911

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Slc18a1/VMAT1 or Slc18a3/VAChT

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Slc18a2 protein

UniProt ID

Q66L40

Shipping

Shipped on ice packs

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Anti-Slc18a2/VMAT2 Antibody (N449/73)

SKU: 75-436

Product Details

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