Anti-Presenilin 1 (PS-1) Antibody

Name: Anti-Presenilin 1 (PS-1) Antibody
Brand: Biosensis
SKU: R-1605-500
Price: 337.0 USD
Availability: InStock

Our Anti-Presenilin 1 (PS-1) rabbit polyclonal primary antibody detects human and mouse Presenilin 1 (PS-1), and is IgG. It is validated for use in WB.

Western Immunoblotting of human and mouse Presenilin 1 protein in mouse cell line extracts and mouse and human brain. Membrane protein load 20 µg. Crude anti-PS1 NT used at 1:2000.

Presenilin 1 detection in PS1 positive and knock-out neurosphere derived cells after differentiation. Immunofluorescence after 4% paraformaldehyde fixation for 15 min, PS1[1-20] protein A-purified (20 µg/mL).

Western Immunoblotting of human and mouse Presenilin 1 protein in mouse cell line extracts and mouse and human brain. Membrane protein load 20 µg. Crude anti-PS1 NT used at 1:2000.

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Species Reactivity

Human, Mouse

Applications

IF, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1605-500

Volume/Size: 500 µg

Variant

Price:

$337.00

Quantity:

Product Details

Target

Presenilin 1 (PS-1)

Scientific Background

Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

IF, WB

Host Species

Rabbit

Molecular Weight

29 kDa, 45 kDa (see application details)

Immunogen

A synthetic peptide corresponding to a region (1-20 aa) from the N-terminus of human Presenilin 1 conjugated to Diptheria toxoid.

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from PBS, pH 7.4. Contains no preservative.

Dilution Ranges

WB: 1:2000
IHC: 1:100
ICC: 1:100

Application Details

On SDS-PAGE, the predominant form detected by this antibody is the N-terminal Presenilin 1 fragment of approx 29 kDa. The uncleaved form of Presenilin 1 migrates to approx 45 kDa. Human and mouse brain samples commonly prepared with reducing agent (50 mM DTT), urea (2.3M), SDS (1%) in 62.5 mM Tris-HCl pH 6.8 sample buffer heated to 50 C for 15 min. The suggested dilution for IF is 1:100 for acetone or paraformaldehyde fixed cells or tissue.

Conjugate

Unconjugated

Specificity

Specificity confirmed by WB and IF using transfected cells, Presenilin 1 knock-out mouse cells, mouse and human brain.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;

UniProt ID

P49768

Shipping

25°C (ambient)

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Anti-Presenilin 1 (PS-1) Antibody

SKU: R-1605-500

Product Details

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