Anti-Autophagy-related protein 7 (ATG7) Antibody

Name: Anti-Autophagy-related protein 7 (ATG7) Antibody
Brand: Biosensis
SKU: R-161-100
Price: 327.0 USD
Availability: InStock

Our Anti-Autophagy-related protein 7 (ATG7) rabbit polyclonal primary antibody detects human and rat Autophagy-related protein 7 (ATG7), and is whole serum. It is validated for use in IHC-Frozen.

Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. Observations: No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line.

Western blot on Peripheral Blood Mononuclear Cells, non-reduced, (PMBC) lysate using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1:100 (ECL).

Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Observations: No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line.

Click on image to zoom

Species Reactivity

Human, Rat

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-161-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Autophagy-related protein 7 (ATG7)

Scientific Background

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:100
IHC: 1:100
ICC: 1:100

Application Details

Non-reduced Western blot : 1:100; IF: acetone or 3.7% PFA fixed cells (HJ Lim et al 2013); 1:100

Conjugate

Unconjugated

Specificity

IF, WB and ELISA confirmed the specificity for ATG7. Human, rat. Other species not yet tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein; hAGP7; APG7-like; ATG7; APG7L

UniProt ID

O95352

Shipping

25°C (ambient)

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Anti-Autophagy-related protein 7 (ATG7) Antibody

SKU: R-161-100

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