Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb FL650 Conjugate (30H3K1)

Name: Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb FL650 Conjugate
Brand: Aves Labs
SKU: 78-605-FL650
Price: 562.0 USD
Availability: InStock

Our chicken Anti-Ubiquitin (Ser65) phosphospecific FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone 30H3K1. It detects human, mouse, and rat Ubiquitin (Ser65) and is purified by affinity chromatography. It works in IHC, ICC.

Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb FL650 Conjugate

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

Conjugates and Formulations

490

550

594

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 78-605-FL650

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ubiquitin (Ser65)

Scientific Background

Ubiquitin phosphorylation at ser65 (pS65-Ub) is implicated in mitochondrial quality control and the molecular origins of Parkinson’s Disease. The protein kinase PINK1 phosphorylates ubiquitin at the ser65 residue in response to mitochondrial stress. This acts as a powerful "on-switch" for the E3 ubiquitin ligase Parkin increasing its enzymatic activity and recruiting it to damaged mitochondria, initiating a specialized degradation process known as mitophagy. When mitochondria are depolarized or damaged, the accumulation of pS65-Ub triggers the clearance of these damaged organelles before they can release toxic reactive oxygen species (ROS) or pro-apoptotic factors. Genetic mutations in either PINK1 or Parkin that disrupt this phosphorylation event are a primary cause of autosomal recessive juvenile Parkinsonism.

Format

Purified by affinity chromatography

Concentration

0.5 mg/mL

Clonality

Recombinant

Clone

30H3K1

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

UBC, UBB

Molecular Weight

Multiple bands

Excitation / Emission

FL650 Ex: 655 nm, Em: 676 nm

Immunogen

The exact immunogen used to generate this antibody is proprietary information

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 30H3K1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 30H3K1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

650

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on mock- and CCCP-treated HeLa lysates and confirmed to stain multiple bands only in CCCP treated lysates

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Polyubiquitin-C, Polyubiquitin-B, pSer65-Ub, Ubiquitin (phospho Ser65),Ser65-phosphorylated ubiquitin

UniProt ID

P0CG48, P0CG47

Shipping

Shipped on ice packs

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Anti-Phospho-Ubiquitin Ser65 Recombinant Chicken Chimeric mAb FL650 Conjugate (30H3K1)

SKU: 78-605-FL650

Product Details

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