Anti-Peripherin Antibody (7C5)
Our Anti-Peripherin mouse monoclonal primary antibody detects cat, human, mouse, other mammals (predicted), and rat Peripherin, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.
![Mixed neuron/glia cultures from newborn rat brain stained with Mouse monoclonal antibody to Peripherin [7C5] (green) and Rabbit polyclonal antibody to NF-L R-1392-50 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both Peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.](http://www.antibodiesinc.com/cdn/shop/files/m-1400-100-ihc_120x120.jpg?v=1759274386)
Mixed neuron/glia cultures from newborn rat brain stained with Mouse monoclonal antibody to Peripherin [7C5] (green) and Rabbit polyclonal antibody to NF-L R-1392-50 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both Peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
Click on image to zoom
SKU: M-1400-100
Product Details
Peripherin
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Affinity-purified
Monoclonal
7C5
IgG
ICC, IHC, WB
Mouse
57 kDa
Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Rat
Feline, Human, Mouse, Rat
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Protein G purified
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
WB: 0.5-2 µg/mL
IHC: 1-5 µg/mL
ICC: 1-5 µg/mL
IHC: 1-5 µg/mL
ICC: 1-5 µg/mL
This antibody performs well on aldehyde fixed tissues.
Unconjugated
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
For research use only.
United States
12 months after date of receipt (unopened vial).
Peripherin; Prph; Prph1;
25°C (ambient)