Anti-Nuclear Pore Complex Protein Nup107 Antibody (39C7)
Our Anti-Nuclear pore complex protein Nup107 mouse monoclonal primary antibody detects human, mouse, rat, and yeast Nuclear pore complex protein Nup107, and is culture supernatant. It is validated for use in ICC.
![Human HeLa Cells grown in tissue culture and stained with Chicken antibody to Vimentin C-1409-50 (red) and Mouse monoclonal antibody to Nuclear Pore Complex [39C7] M-1397-250 (green). The Vimentin filaments in the cytoplasm of the cells are clearly visible in red, while the nuclear pore complex is also visible in green. The 39C7 antibody recognizes nuclear pore complexes in all eukaryotes tested to date. Picture taken with a Zeiss 40X objective and documented with a Digital SPOT camera.](http://www.antibodiesinc.com/cdn/shop/files/m-1397-250-ihc_120x120.jpg?v=1759274381)
![Left: Analysis of nuclear pore complex (NPC) expression in HeLa cells by Immunocytochemistry. Cells were stained with pan-specific mouse anti-NPC antibody (red, 1:100), and co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. The NPC antibody reveals strong granular staining of the nuclei corresponding to the localization of NPC, while the vimentin antibody labels intermediate filaments in these cells. Right: Western blot analysis of cell lysates for NPC expression (green, lanes 2-7, 1:100), using cytosol- or nuclear-enriched fractions. [1] protein standard, [2] HEK293 cytosol, [3] HEK293 nuclear, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear, [6] HeLa cytosol, and [7] HeLa nuclear fraction lysate. The band at about 68 kDa represents a currently unidentified NPC protein which is detected predominantly in the nuclear enriched fractions of all cell lines.](http://www.antibodiesinc.com/cdn/shop/files/m-1397-250-ihc-wb_120x120.jpg?v=1759274381)
Human HeLa Cells grown in tissue culture and stained with Chicken antibody to Vimentin C-1409-50 (red) and Mouse monoclonal antibody to Nuclear Pore Complex [39C7] M-1397-250 (green). The Vimentin filaments in the cytoplasm of the cells are clearly visible in red, while the nuclear pore complex is also visible in green. The 39C7 antibody recognizes nuclear pore complexes in all eukaryotes tested to date. Picture taken with a Zeiss 40X objective and documented with a Digital SPOT camera.
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SKU: M-1397-250
Product Details
Nuclear pore complex protein Nup107
The Nuclear Core Complex (NPC) acts as a gateway for macromolecular traffic between the cytoplasm and the nucleus.
Culture supernatant
Monoclonal
39C7
IgG1
ICC
Mouse
Yeast nuclear preparation
Yeast
Human, Mouse, Rat, Yeast
Spin the vial briefly before opening it. Reconstitute in 250 uL sterile-filtered, room temperature, ultrapure water. Let the vial rehydrate for 5 minutes. Mix gently with a pipette tip to help the redissolution of the material. Centrifuge to remove any insoluble material. The final solution will contain no preservatives. A sterile technique is recommended.After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Concentrated cell culture supernatant.
Lyophilized hybridoma cell culture media with sodium azide.
ICC: 1:50-1:500
This antibody does not work well for Western blotting.
Unconjugated
The specificity of this antibody has been confirmed by IC. Hu, rat, Ms, Yeast.
For research use only.
United States
12 months after date of receipt (unopened vial).
Nuclear pore complex protein Nup107; 107 kDa nucleoporin; Nucleoporin Nup107; NUP107;
25°C (ambient)