Anti-Neuronal nuclei antigen NeuN/FOX3 Antibody
Our Anti-Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3) rabbit polyclonal primary antibody detects human, mouse, and rat Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3), and is affinity-purified. It is validated for use in IF, ICC, IHC, WB.
![Image of a rat brain section by Immunofluorescence and Immunohistochemistry. The section was stained with R-3771-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (red, 1:2,000), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (green, 1:5,000). Product R-3771-100 selectively stains nuclei and cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.](http://www.antibodiesinc.com/cdn/shop/files/r-3771-100-ihc-1_120x120.jpg?v=1759274886)
![Analysis by western blot of NeuN/FOX3 expression in whole brain lysates, cytosolic (cyt) and nuclear enriched (nuc) with R-3771-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (green, 1:1,000) with two bands at approx 46kDa and 48kDa corresponding to the two alternate transcripts of the of NeuN/FOX3 protein. Lane 1: protein standard (red); Lane 2: rat cyt; Lane 3:rat nuc; Lane 4:mouse cyt; Lane 5: mouse nuc lysate. Blot performed under non-reducing conditions.](http://www.antibodiesinc.com/cdn/shop/files/r-3771-100-wb_120x120.jpg?v=1759274886)
![Image of formalin-fixed paraffin-embedded mouse hippocampus section by Immunohistochemistry. The section was stained with R-3771-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (brown, 1:4,000). Product R-3771-100 selectively stains the nuclei and distal perikaryal of most neuronal cell populations.](http://www.antibodiesinc.com/cdn/shop/files/r-3771-100-ihc-2_120x120.jpg?v=1759274886)
![Image of a rat brain section by Immunofluorescence and Immunohistochemistry. The section was stained with R-3771-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (red, 1:2,000), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (green, 1:5,000). Product R-3771-100 selectively stains nuclei and cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.](http://www.antibodiesinc.com/cdn/shop/files/r-3771-100-ihc-1_1600x.jpg?v=1759274886)
Image of a rat brain section by Immunofluorescence and Immunohistochemistry. The section was stained with R-3771-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Rabbit pAb, (red, 1:2,000), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (green, 1:5,000). Product R-3771-100 selectively stains nuclei and cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.
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