Anti-mCherry Antibody

Name: Anti-mCherry Antibody
Brand: Biosensis
SKU: R-1654-100
Price: 327.0 USD
Availability: InStock

Our Anti-mCherry rabbit polyclonal primary antibody detects mCherry, and is affinity-purified. It is validated for use in ICC, WB.

Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit antibody to mCherry (green, 1:1,000). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with a full length GFP construct. A major band at about 28 kDa corresponds to mCherry protein. The antibody does not cross-react with GFP protein. The same blot was simultaneously probed with a chicken antibody to HSP60 (red, lanes 2-6).

Anti-mCherry Antibody

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Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1654-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

mCherry

Scientific Background

mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Foerster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

~28-30 kDa

Immunogen

Recombinant full length mCherry.

Immunogen Species

Jellyfish

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:250-1:500

Conjugate

Unconjugated

Specificity

The antibody reacts with a band at ~28-30 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

X5DSL3

Shipping

25°C (ambient)

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Anti-mCherry Antibody

SKU: R-1654-100

Product Details

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