Anti-MANF Antibody

Name: Anti-MANF Antibody
Brand: Biosensis
SKU: R-1675-100
Price: 457.0 USD
Availability: InStock

Our Anti-Mesencephalic astrocyte-derived neurotrophic factor (MANF) rabbit polyclonal primary antibody detects human Mesencephalic astrocyte-derived neurotrophic factor (MANF), and is affinity-purified. It is validated for use in WB.

Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85<cell line.

Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85 Click on image to zoom

Species Reactivity

Human

Applications

Host Species

Rabbit

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Data Sheet

SKU: R-1675-100

Volume/Size: 100 µg

Variant

Price:

$457.00

Quantity:

Product Details

Target

Mesencephalic astrocyte-derived neurotrophic factor (MANF)

Scientific Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Immunogen

Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.

Immunogen Species

Human

Species Reactivity

Human

Storage

Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250 mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life. Store antibody stock solution at 2-8°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.

Physical State

Liquid

Buffer

Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.

Dilution Ranges

WB: 1:2000-1:6000

Conjugate

Unconjugated

Specificity

MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

ARMET, ARP

UniProt ID

P55145

Shipping

2-8°C (on cold packs)

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Anti-MANF Antibody

SKU: R-1675-100

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