Anti-Fibronectin Antibody

Name: Anti-Fibronectin Antibody
Brand: Antibodies Incorporated
SKU: RP-4003
Price: 337.0 USD
Availability: InStock

Our rabbit Fibronectin polyclonal antibody from Antibodies Incorporated is produced in-house. It detects human Fibronectin and is purified by Protein A chromatography. Works in IHC, ICC, WB, ELISA.

Immunofluorescent staining of HepG2 cells using 5 ug/mL rabbit anti-fibronectin antibody (RP-4003) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-fibronectin stains the membrane of HepG2 cells. ferritin in the cytoplasm of HepG2 cells.

Western blotting of HepG2 cell lysate using 0.2 ug/mL, 1 ug/mL, or 5 ug/mL Antibodies Incorporated rabbit anti-fibronectin antibody (RP-4003). RP-4003 rabbit anti-fibronectin antibody recognizes endogenous fibronectin at 200 kDa.

Immunofluorescent staining of HepG2 cells using 5 ug/mL rabbit anti-fibronectin antibody (RP-4003) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-fibronectin stains the membrane of HepG2 cells. ferritin in the cytoplasm of HepG2 cells.

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Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: RP-4003

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Fibronectin

Scientific Background

Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimers of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

FN1

Molecular Weight

200-220 kDa

Immunogen

Purified human Fibronectin from human plasma

Immunogen Species

Human

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by Protein A affinity chromatography.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000 - 1:5000
IHC: 1:100 - 1:200
ICC: 1:100 - 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

FN1; FN

UniProt ID

P02751

Shipping

Shipped on ice packs

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Anti-Fibronectin Antibody

SKU: RP-4003

Product Details

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