Anti-FHOD1 (Thr-1141), Phosphospecific Antibody

Name: Anti-FHOD1 (Thr-1141), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: FP3481
Price: 340.0 USD
Availability: InStock

6 Citations

Our FHOD1 (Thr-1141) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat FHOD1 (Thr-1141) and is antigen affinity purified. It is great for use in WB.

Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .

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Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

SKU: FP3481

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FHOD1 (Thr-1141)

Scientific Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

FHOD1

Molecular Weight

140

Immunogen

Phospho-FHOD1 (Thr-1141) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-1141 in human FHOD1. This peptide sequence is highly conserved in rat and mouse FHOD1, and is well conserved in FHOD3 (Thr-1399).

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated FHOD1 (Thr-1141) peptide before affinity purification using phospho-FHOD1 (Thr-1141) peptide (without carrier). The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and mouse C2C12 cells treated with calyculin A.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-1141

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FHOS, formin

UniProt ID

Q9Y613

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
29885024	not listed	Boddy, K.C., et al. 2018. Septin-regulated actin dynamics promote Salmonella invasion of host cells. Cell Microbiology, e12866.
23869992	not listed	Truong, D., et al. 2013. Formin-mediated actin polymerization promotes Salmonella invasion. Cell Microbiology, 2051-2063.

Western Blot: Mouse
PMID	Dilution	Publication
29396114	not listed	Pandey, P, et al. 2018. Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions. Developmental cell, 326-336.
25125170	not listed	Dwyer, J., et al. 2014. The formin FHOD1 in cardiomyocytes. Anatomical Recort, 1560-1570.
24331927	not listed	Iskratsch, T., et al. 2013. FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. Developmental cell, 545-559.
21106951	not listed	Staus, D.P., et al. 2011. Formin homology domain-containing protein 1 regulates smooth muscle cell phenotype. Arterioschlerosis, Thrombosis, and Vascular Biology, 360-367.

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Anti-FHOD1 (Thr-1141), Phosphospecific Antibody

SKU: FP3481

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-FHOD1 (Thr-1141), Phosphospecific Antibody

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