Anti-FHOD1 Antibody (M352)

Name: Anti-FHOD1 Antibody
Brand: PhosphoSolutions
SKU: FM3521
Price: 285.0 USD
Availability: InStock

7 Citations

Our FHOD1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human FHOD1 and is Protein A purified. It is great for use in WB, IHC.

Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .

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Species Reactivity

Human

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

SKU: FM3521

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FHOD1

Scientific Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M352

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Gene Name

FHOD1

Molecular Weight

140

Immunogen

FHOD1 recombinant protein that included the full length human FHOD1 sequence. This sequence has 50% homology to human FHOD3, and low homology to other formin family members.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
IHC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and Jurkat, and has weak reactivity to FHOD1 in mouse C2C12.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FHOS, formin

UniProt ID

Q9Y613

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
26621033	1:50	Panzer, L., et al. 2016. The formins FHOD1 and INF2 regulate inter- and intra-structural contractility of podosomes. Journal of Cell Science, 298-313.
23869992	not listed	Truong, D., et al. 2013. Formin-mediated actin polymerization promotes Salmonella invasion. Cell Microbiology, 2051-2063.

Immunohistochemistry: Human
PMID	Dilution	Publication
31104842	not listed	Kalappurakkal, JM, et al. 2019. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell, 1738-1756.

Western Blot: Human
PMID	Dilution	Publication
29885024	not listed	Boddy, K.C., et al. 2018. Septin-regulated actin dynamics promote Salmonella invasion of host cells. Cell Microbiology, e12866.
24481812	1:200	Schulze, N., et al. 2014. FHOD1 regulates stress fiber organization by controlling the dynamics of transverse arcs and dorsal fibers. Journal of Cell Science, 1379-1393.
23869992	not listed	Truong, D., et al. 2013. Formin-mediated actin polymerization promotes Salmonella invasion. Cell Microbiology, 2051-2063.

Western Blot: Mouse
PMID	Dilution	Publication
31104842	not listed	Kalappurakkal, JM, et al. 2019. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell, 1738-1756.
21106951	not listed	Staus, D.P., et al. 2011. Formin homology domain-containing protein 1 regulates smooth muscle cell phenotype. Arterioschlerosis, Thrombosis, and Vascular Biology, 360-367.

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Anti-FHOD1 Antibody (M352)

SKU: FM3521

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-FHOD1 Antibody

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