Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Name: Anti-DYKDDDDK Epitope Tag/FLAG Antibody
Brand: Aves Labs
SKU: ET-DY100
Price: 311.0 USD
Availability: InStock

Our Anti-DYKDDDDK Epitope Tag/FLAG Antibody chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a DYKDDDDK Epitope Tag/FLAG Antibody, and is affinity-purified IgY. Works in ELISA, IHC, ICC, IP, WB.

Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.

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Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

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Data Sheet

SKU: ET-DY100

Volume: 1 mL

Variant

Price:

$311.00

Quantity:

Ships: 1-2 business days

Product Details

Target

DYKDDDDK Epitope Tag/FLAG Antibody

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Molecular Weight

N/A

Immunogen

Synthetic peptide

Antibody Registry ID

AB_2313510

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

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Anti-DYKDDDDK Epitope Tag/FLAG Antibody

SKU: ET-DY100

Product Details

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