Anti-Cytokeratin 8 Recombinant Chicken Chimeric mAb (E8-C6-B4)

Name: Anti-Cytokeratin 8 Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-602
Price: 470.0 USD
Availability: InStock

Our Anti-Cytokeratin 8 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone E8-C6-B4. It detects human and bovine Cytokeratin 8, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC.

Immunofluorescent staining of HeLa cells using Aves Labs anti-Cytokeratin 8 (CK8) recombinant chicken monoclonal antibody (green). The stained cells were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-CK8 specifically stains the cytoskeleton of HeLa cells.

Western blotting of HeLa cell lysates with Aves Labs anti-Cytokeratin 8 (CK8) recombinant chicken mAb antibody at ~52 kDa. It was detected with anti-chicken-HRP (Cat No. 80-1000-HRP).

Immunofluorescent staining of HeLa cells using Aves Labs anti-Cytokeratin 8 (CK8) recombinant chicken monoclonal antibody (green). The stained cells were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-CK8 specifically stains the cytoskeleton of HeLa cells.

Click on image to zoom

Species Reactivity

Bovine, Human

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: 78-602

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Cytokeratin 8

Scientific Background

Cytokeratin 8 (KRT8) is a type II intermediate filament protein of ~52 kDa that pairs with cytokeratin 18 to form heteropolymers in simple epithelial cells. Together, these keratins provide structural stability to epithelial tissues and contribute to cellular resilience under mechanical and apoptotic stress. Beyond their cytoskeletal role, KRT8 and KRT18 participate in intracellular signaling, apoptosis regulation, and stress response pathways. Abnormal expression or post-translational modification of KRT8 has been linked to a variety of human diseases, including chronic liver disease, inflammatory disorders, and multiple carcinomas. Because of its restricted expression in simple epithelia and frequent upregulation in tumors, KRT8 serves as both a diagnostic marker and a functional mediator of epithelial cell biology and disease progression.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

E8-C6-B4

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

KRT8

Molecular Weight

52 kDa

Immunogen

C-terminal amino acids 472-483 of Human Cytokeratin 8

Immunogen Species

Human

Species Reactivity

Bovine, Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone E8-C6-B4 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone E8-C6-B4 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000-1:3000
IHC: 1:500-1:2000
ICC: 1:500-1:2000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Keratin-8, CK-8, Type-II keratin Kb8

UniProt ID

P05787

Shipping

Shipped on ice packs

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Anti-Cytokeratin 8 Recombinant Chicken Chimeric mAb (E8-C6-B4)

SKU: 78-602

Product Details

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