Anti-C-MYC Epitope Tag Antibody

Name: Anti-C-MYC Epitope Tag Antibody
Brand: Aves Labs
SKU: ET-MY100
Price: 311.0 USD
Availability: InStock

This product is an unlabeled, affinity-purified chicken anti-C-MYC epitope antibody.

Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.

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Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

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Data Sheet

SKU: ET-MY100

Volume: 1 mL

Variant

Price:

$311.00

Quantity:

Ships: 1-2 business days

Product Details

Target

C-MYC Epitope Tag Antibody

Scientific Background

The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL).

Format

Affinity Purified IgY

Concentration

100 µg/mL (based on absorbance at 280 nm)

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Molecular Weight

N/A

Immunogen

Synthetic peptide

Antibody Registry ID

AB_2313514

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:100-1:250
ICC: 1:100-1:250

Quality Control

Antibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

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Anti-C-MYC Epitope Tag Antibody

SKU: ET-MY100

Product Details

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