Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

Name: Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody
Brand: Aves Labs
SKU: BGL-1040
Price: 311.0 USD
Availability: InStock

Our Beta-Galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!

A tissue section through an e18 mouse embryo showing β-galactosidase immunostaining (green staining) in the otic vesicle and adjacent otic ganglion. Hoechst dye (purple staining) shows the nuclei of cells.

Basolateral amygdala from c-fos-lacZ transgenic male rats labeling LacZ (Cat. BGal, 1:1000, green). The image was kindly provided by Benedetto Sacchetti, Università di Torino.

Transgenic rats that received vehicle (left panel) or Daun02 (right panel) were perfused 90 min after the last CS1 presentation and analyzed for the expression of β-galactosidase (β-gal) (Cat. BGL, 1:1000). Image from publication CC-BY-4.0. PMID:38656872

A tissue section through an e18 mouse embryo showing β-galactosidase immunostaining (green staining) in the otic vesicle and adjacent otic ganglion. Hoechst dye (purple staining) shows the nuclei of cells.

Click on image to zoom

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: BGL-1040

Volume: 400 µL

Variant

Price:

$311.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Beta-Gal (Beta-Galactosidase, LacZ)

Scientific Background

Beta Galactosidase is the product of the LacZ gene and is widely used as a reporter marker to monitor gene expression.

Format

Mixture of IgY fraction and affinity-purified antibodies

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

117 kDa

Immunogen

Native protein expressed Escherichia coli

Antibody Registry ID

AB_2313508

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified ß-Galactosidase (lacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), and is filter-sterilized.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%,w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2500-1:5000
ICC: 1:2500-1:5000

Quality Control

This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005).

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

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Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

SKU: BGL-1040

Product Details

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