Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (Cat. ET-HA) fused to their N-termini. Actin (Cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
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Human, Mouse, Rat
WB
Chicken

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Data Sheet

SKU: ACT-1040

Volume: 400 µL
Price:
$311.00
Ships: 1-2 business days

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