Anti-Presenilin 1 loop region Antibody

Name: Anti-Presenilin 1 loop region Antibody
Brand: Biosensis
SKU: R-1680-500
Price: 347.0 USD
Availability: InStock

Our Anti-Presenilin 1 loop region rabbit polyclonal primary antibody detects human and mouse Presenilin 1 loop region, and is IgG. It is validated for use in WB.

Western Immunoblotting of mouse and human Presenilin 1 protein in mouse cell line extracts, various mouse tissues and a human cell line. Membrane proteins were prepared and loaded as 20 µg protein per lane. Crude anti-PS1 loop antibody used at 1:1000.

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Species Reactivity

Human, Mouse

Applications

Host Species

Rabbit

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Data Sheet

SKU: R-1680-500

Volume/Size: 500 µg

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Presenilin 1 loop region

Scientific Background

Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Molecular Weight

19 kDa, 45 kDa (See application details.)

Immunogen

A synthetic peptide (GDPEAQRRVSKNSKYNA-C) corresponding to human PS1 [301-317] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from PBS, pH 7.4. Contains no preservative.

Dilution Ranges

WB: 1:1000

Application Details

Full length presenilin 1 (467 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 19 kDa. Human or mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min.

Conjugate

Unconjugated

Specificity

Confirmed by Western blotting using transfected cells, presenilin 1 knock-out mouse cells and mouse and human brain.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

ACNINV3; Presenilin 1; FAD; PS-1; Protein S182; PS1; PS1-CTF12; S182; PSEN1; presenilin-1; AD3; presenilin-1 isoform I-467; PSNL1

UniProt ID

P49768
UniProt Summary: Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity. Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling. Required for normal embryonic brain and skeleton development, and for normal angiogenesis. Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis. Involved in the regulation of neurite outgrowth. Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression.

NCBI Gene ID

5663

Shipping

25°C (ambient)

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Anti-Presenilin 1 loop region Antibody

SKU: R-1680-500

Product Details

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