Anti-PARP2 Antibody

Name: Anti-PARP2 Antibody
Brand: PhosphoSolutions
SKU: 24280
Price: 456.0 USD
Availability: InStock

Our PARP2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human PARP2 and is antigen affinity purified. It is great for use in WB.

Western Blot of 3T3 cell lysate showing specific immunolabeling of PARP2.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: 24280

Title: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PARP2

Scientific Background

Poly (ADP-ribose) polymerase 1, (PARP-1) and poly (ADP-ribose) polymerase 2 (PARP-2) catalyze the formation of poly (ADP-ribose) polymers in a DNA-dependent manner. PARP-2 displays automodification properties similar to PARP-1. These act by poly (ADP-ribosylation), which is a post-translational modification of nuclear proteins in response to DNA damage that activates the base excision repair machinery.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

PARP2

Immunogen

Synthetic peptide corresponding to amino acids from the N-terminal region of human PARP-2 protein

Immunogen Species

Human

Species Reactivity

Human

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000

Specificity

Reacts with the N-terminal region of the 62 kDa human PARP-2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ADPRT2; ADP ribosyltransferase like 2; ADPRTL3; ARTD2; PARP-2; pADPRT-2; poly [ADP-ribose polymerase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT-2; NAD(+ ADP-ribosyltransferase 2; hPARP-2; poly (ADP-ribose polymerase family; member 2; poly (ADP-ribosyl transferase-like 2; poly[ADP-ribose synthase 2; poly[ADP-ribose synthetase 2; Poly ADP-ribose polymerase 2; DNA ADP-ribosyltransferase PARP2; Protein poly-ADP-ribosyltransferase PARP2

UniProt ID

Q9UGN5
UniProt Summary: Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains. Specifically mediates formation of branched poly-ADP-ribosylation. Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex.

NCBI Gene ID

10038

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Anti-PARP2 Antibody

SKU: 24280

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