Anti-Myristoylated alanine rich C kinase substrate (MARCKS) Antibody

Name: Anti-Myristoylated alanine rich C kinase substrate (MARCKS) Antibody
Brand: Biosensis
SKU: R-1380-50
Price: 337.0 USD
Availability: InStock

Our Anti-Myristoylated alanine rich C kinase substrate (MARCKS) rabbit polyclonal primary antibody detects human Myristoylated alanine rich C kinase substrate (MARCKS), and is affinity purified. It is validated for use in ICC, WB.

Image of mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS) R-1380-50 (red) and Chicken polyclonal antibody to MAP2 C-1382-50. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.

Left: Analysis of MARCKS expression (red) in HeLa cells by Immunocytochemistry. MARCKS protein was detected with rabbit antibody to MARCKS at 1:1,000 dilution. Cells were co-stained with a mouse anti-beta tubulin antibody (green). Blue: DAPI nuclear stain. The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm. Right: Western blot analysis of MARCKS expression (green) in various cell line lysates using rabbit anti-MARCKS antibody (1:1,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6. The blot was probed simultaneously with mouse antibody to GAPDH (M-1376-250, red, 1:5,000). The strong band at 80 kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells (lane 5), but no reactivity is seen on rodent cells (lanes 6 and 7). The GAPDH antibody used as a loading control reveals a single band at 37 kDa in all preparations.

Image of mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS) R-1380-50 (red) and Chicken polyclonal antibody to MAP2 C-1382-50. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1380-50

Volume/Size: 50 µg

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Myristoylated alanine rich C kinase substrate (MARCKS)

Scientific Background

Myristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.

Format

Affinity Purified

Clonality

Polyclonal

Isotype

Mixed

Applications

ICC, WB

Host Species

Rabbit

Immunogen

Recombinant full length MARCKS expressed and purified from E. coli

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:10000-1:20000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. Does not react with rodent protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

80 kDa protein; Myristoylated alanine-rich C-kinase substrate; 80K L; MARCKS; 80K L protein; Protein kinase C substrate 80 kDa protein; 80K-L protein; Macs; 80KL ,81 kDa protein; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS; Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate

UniProt ID

P30009
UniProt Summary: Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes. Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton. Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane.

NCBI Gene ID

4082

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Anti-Myristoylated alanine rich C kinase substrate (MARCKS) Antibody

SKU: R-1380-50

Product Details

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