Anti-Autophagy protein 5 (ATG5) Antibody

Name: Anti-Autophagy protein 5 (ATG5) Antibody
Brand: Biosensis
SKU: R-138-500
Price: 337.0 USD
Availability: InStock

Our Anti-Autophagy protein 5 (ATG5) rabbit polyclonal primary antibody detects human Autophagy protein 5 (ATG5), and is IgG. It is validated for use in IHC-Frozen, WB.

Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG5 (cat # R-138-500, 2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-138-500.

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Species Reactivity

Human

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-138-500

Volume/Size: 500 µg

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Autophagy protein 5 (ATG5)

Scientific Background

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 500 µL of sterile-filtered, ultra pure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from a solution containing PBS pH 7.4

Dilution Ranges

WB: 1:200-1:1000
IHC: 1:200-1:1000
ICC: 1:200-1:1000

Application Details

Flow Cytometry (2 ug/ 10⁶ cells)

Conjugate

Unconjugated

Specificity

IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

APG5; Autophagy protein 5; APG5L; APG5-like; ASP; APG 5; SCAR25; hAPG5; Apoptosis-specific protein; ATG5; APG5 autophagy 5-like; ATG5 autophagy related 5 homolog

UniProt ID

Q9H1Y0
UniProt Summary: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress.

NCBI Gene ID

9474

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25°C (ambient)

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Anti-Autophagy protein 5 (ATG5) Antibody

SKU: R-138-500

Product Details

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