Anti-MeCP2 (Ser-421), Phosphospecific Antibody

Name: Anti-MeCP2 (Ser-421), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: MP4611
Price: 340.0 USD
Availability: InStock

5 Citations

Our MeCP2 (Ser-421) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MeCP2 (Ser-421) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of human PC3 cells treated with calyculin A (25 mM) for 15 min. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (C-terminus) (lanes 1 & 2) or anti-MeCP2 (Ser-421) (lanes 3 & 4).

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-421) rabbit polyclonal antibody (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot of human PC3 cells treated with calyculin A (25 mM) for 15 min. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (C-terminus) (lanes 1 & 2) or anti-MeCP2 (Ser-421) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: MP4611

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MeCP2 (Ser-421)

Scientific Background

Methyl-CpG Binding Protein 2 (MeCP2) was identified based on its affinity for methylated cytosines within DNA. As a chromatin-associated multifunctional protein, MeCP2 has been implicated in regulation of transcription and chromatin structure. Mutations of MeCP2 cause Rett syndrome, which results from neuronal dysfunction and impairment in cognitive and motor functions. Regulation of MeCP2 activity may involve phosphorylation at multiple sites. Ser-421 in MeCP2 is phosphorylated in response to neuronal activity, calcium influx, and is dependent on Cam-KII. Alanine mutation of Ser-421 leads to defects in synapse development and activity. Ser-80 in MeCP2 is phosphorylated in HeLa nuclear extracts and neurons. Alanine mutation of Ser-80 attenuates MeCP2 chromatin association and leads to locomotor deficits in transgenic knock-in mice. Thus, phosphorylation of MeCP2 may be important for altering its function during neuronal activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MECP2

Molecular Weight

Immunogen

phospho-MeCP2 (Ser-421) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-421

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Methyl-CpG-Binding2

UniProt ID

Q9Z2D6

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunohistochemistry: Rat
Western Blot: Mouse | Rat

Immunohistochemistry: Rat
PMID	Dilution	Publication
27493805	1:200	Muñoz, P, et al. 2016. Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats. Neural Plasticity, 4783836.

Western Blot: Mouse
PMID	Dilution	Publication
34183865	1:1000	Kim, JW, et al. 2021. Sustained effects of rapidly acting antidepressants require BDNF-dependent MeCP2 phosphorylation. Nature neuroscience, 1100-1109.

Western Blot: Rat
PMID	Dilution	Publication
32898508	1:500	Grannonico, M, et al. 2020. DF3016A induces increased BDNF transcription in ischemic neuroinflammation injury. Brain research, 147057.
30209341	1:1000	Lee, J., et al. 2018. The acetylation of cyclin-dependent kinase 5 at lysine 33 regulates kinase activity and neurite length in hippocampal neurons. Scientific Reports, 13676.
24718660	1:1000	Wang, A., et al. 2014. Epigenetic upregulation of corticotrophin-releasing hormone mediates postnatal maternal separation-induced memory deficiency. PLoS One, e94394.

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Anti-MeCP2 (Ser-421), Phosphospecific Antibody

SKU: MP4611

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-MeCP2 (Ser-421), Phosphospecific Antibody

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