PhosphoSolutions

968 products

PhosphoSolutions’ Antibodies that Work™ have been trusted by researchers since 2001. With a focus in both neuroscience and cancer research, we pride ourselves on offering the highest quality antibodies and customer support in the business. Our serum-pooling initiative ensures reproducible polyclonal antibodies that come with a 100% satisfaction guarantee. Our all-inclusive custom antibody services allow you to work directly with the scientist developing your antibody: from design and immunization to purification and characterization. We understand how frustrating it can be to conduct research using ineffective antibodies, and we believe that you shouldn’t have to.

Showing 121 - 144 of 968 products

Western blot analysis of adult mouse diaphram treated with thapsigargin for 0, 6, 12, or 24 hours to induce cleavage of αII-spectrin from 250 kDa to 136 kDa. The blot was probed with rabbit polyclonal αII-spectrin (a.a. 1171-1176) cleavage-specific antibody at a dilution of 1:1,000 (Image provided by Dr. Leigh Ann Callahan, Department of Internal Medicine, University of Kentucky.)

PhosphoSolutions Anti-αII-Spectrin , cleavage-specific Antibody

$348

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118)
phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-Sox2 (Thr-118), Phosphospecific Antibody

$348

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).

PhosphoSolutions Anti-SHP1 (Tyr-536), Phosphospecific Antibody

$348

Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).

PhosphoSolutions Anti-PTP1B (Tyr-152), Phosphospecific Antibody

$348

Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).

PhosphoSolutions Anti-Paxillin (Tyr-118), Phosphospecific Antibody

$348

Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).

Formalin fixed, citric acid treated parafin sections of adult Rat striatum. Sections were probed with anti-nNOS (NP2141) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

PhosphoSolutions Anti-nNOS (C-terminal region) Antibody

$292

Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1µg/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).

Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-iNOS (Tyr-1055) [conserved site], Phosphospecific Antibody

$348

Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).

PhosphoSolutions Anti-Myosin IIA Heavy Chain (Ser-1916), Phosphospecific Antibody

$348

Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).

PhosphoSolutions Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

$292

Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).

PhosphoSolutions Anti-MuRF1 (C-terminal region) Antibody

$348

Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).

PhosphoSolutions Anti-Histone H2B (N-terminal region) Antibody

$348

Immunocytochemical labeling of Histone H2B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B (C-terminus) antibody (HP4291), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-Histone H2B (C-terminus) Antibody

$348

Western blot of human recombinant DDR1 protein with C-terminal His Tag. The blot was probed with mouse monoclonal anti-His (Cterminal) Tag (HM0501) at 1:2000 (lane 1), 1:4000 (lane 2), and 1:8000 (lane 3).

Immunocytochemical labeling of Axl recombinant protein with C-terminal His Tag bound to THP1 aldehyde fixed cells. The cells were labeled with mouse monoclonal anti-His (C-terminal) Tag antibody (HM0501). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-His (C-terminal) Tag Antibody

$292

Paraformaldehyde-fixed zebrafish embryos were probed with anti-EphA4 (Tyr-602) (EP2731) then detected using Alexa Fluor 647 goat anti-rabbit. Arrows show labeling of somite boundaries and the notochord. (Image provided by Dr. Scott Holley at the Department of Molecular, Cellular and Developmental Biology, Yale University.)

Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

PhosphoSolutions Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody

$348

Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

PhosphoSolutions Anti-eEF2K (Ser-359), Phosphospecific Antibody

$348

Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with γ-Catenin (a.a. 545-555), γ-Catenin (Tyr-550) or γ-Catenin (C-terminal) antibodies. The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) (lane 5) or γ-Catenin (Tyr-644) (lane 6) peptides.

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.

PhosphoSolutions Anti-γ-Catenin (Tyr-550), Phosphospecific Antibody

$348

Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).

PhosphoSolutions Anti-Atrogin-1 Antibody

$348

Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.

Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)

PhosphoSolutions Anti-Asc (Tyr-144), Phosphospecific Antibody

$348

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).

Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.

PhosphoSolutions Anti-Arp2 (C-terminal region) Antibody

$348

Immunofluorescence of healthy human kidney. The antibody specifically labels pre-pro-vasopressin (white) in the collecting ducts (DBA, red), but not in the proximal tubules (LTL, green) of human kidney. DNA is labeled with DAPI (blue). Image kindly provided by JP Arroyo, Vanderbilt University.

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~20 kDa uncleaved pre-pro vasopressin protein.

PhosphoSolutions Anti-Pre-Pro-Vasopressin

From $164

Immunofluorescence of transfected COS-7 cells specifically labeling the positive SARM1 cells using our anti-SARM1 antibody (1:50, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).

Western blot of HeLa cell lysate showing specific immunolabeling of the ~79 kDa SARM1 protein.

PhosphoSolutions Anti-SARM1 Antibody

From $164

Western blot of rat whole brain lysate showing specific immunolabeling of the ~35 kDa AQP4 protein.

PhosphoSolutions Anti-Aquaporin 4 Antibody

From $164

$Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).$

PhosphoSolutions Anti-p53 (Ser392) Antibody

From $164

Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

PhosphoSolutions Anti-MEK5 (Ser311, Thr315) Antibody

From $164

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