PhosphoSolutions

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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
PhosphoSolutions Anti-Dopamine Transporter, Extracellular Loop 2 Antibody
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Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 (Cat. 374-DARPP, green, 1:250) and Tuj1(red). Cells and photo courtesy of QBM CellScience.Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein.
PhosphoSolutions Anti-DARPP-32 Antibody
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Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP (Cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.Western blot of rat brain lysate showing the specific immunolabeling of the ~46 kDa CNP protein.
PhosphoSolutions Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
$385
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR1 Subunit Antibody
$445
Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu. Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit, N-terminus Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit. Immunostaining of 21 DIV nucleofected mouse striatal neuron (green) co-cultured with cortical neurons showing nice punctate labeling of the N-terminal NR2A subunit (red, 1:500) in both the medium spiny neurons and the large pyramidal cell in the upper right. Photo courtesy of Dr. A.J. Milnerwood, Dr. Lynn Raymond Lab, University of British Columbia.
PhosphoSolutions Anti-NMDA NR2A Subunit, N-terminus Antibody
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Western blot of mouse habenula lysate showing specific immunolabeling of the ~52 kDa nAChRβ2 protein.
PhosphoSolutions Anti-Nicotinic Acetylcholine Receptor (nAChR) β2 Antibody
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Immunofluorescent image of mouse retina specifically labeling arginase (Cat. 146-ARG, 1:500, green) and CD206 (red). The blue nuclear stain is DAPI. The image was kindly provided by Sarah Gough, University of Miami Miller School of Medicine.Western blot of mouse liver lysate showing specific immunolabeling of the ~35, 38 kDa arginase I protein.
PhosphoSolutions Anti-Arginase I (ARG1) Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet. Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (Cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (Cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
PhosphoSolutions Anti-Nestin Antibody
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Western blot of mouse cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.Immunostaining of rat hippocampus showing specific labeling of neuronal dendrites and perikarya with Anti-Microtubule Associated Protein 2 in green, FOX3 in red and nuclei in blue.
PhosphoSolutions Anti-Microtubule Associated Protein 2 (MAP2) Antibody
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Immunofluorescence of a section of rat cerebellum selectively labeling the axons of granule cells with alpha-internexin (Cat. 100-AIN, 1:5000, green) and colabeled with anti-calretinin (red).Immunofluorescence of cultured rat CNS cells showing specific labeling of neuronal processes with Anti-alpha-internexin (Cat. 100-AIN, 1:250, red), and specific labeling of perikarya and dendrites with anti-microtubule associated protein 2 (Cat. 1100-MAP2,1:2,500,green).
PhosphoSolutions Anti-Alpha Internexin/NF-66 Antibody
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Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.Immunostaining of fibrotic mouse lung tissue showing specific staining of collagen I molecules (Cat. 621-COLP, 1:100, red) that are still associated with the cells in which they were synthesized. The blue is staining DNA.
PhosphoSolutions Anti-Collagen 1, alpha 1 propeptide Antibody
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Immunohistochemical staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein (red, 1;100). Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.Western blot of 70 day old mouse retina lysate showing immunolabeling of the ~220 kDa ABCA4 protein at a dilution of 1:1000.
PhosphoSolutions Anti-ABCA4 (Rim Protein) Antibody
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
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Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained and photo was provided courtesy of QBMCellScience.
PhosphoSolutions Anti-Calcitonin Gene Related Peptide (CGRP) Antibody
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Immunofluorescence of HeLa cells labeled with anti-mitochondria antibody (1:100, green).Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa mitochondrial protein.
PhosphoSolutions Anti-Mitochondria Antibody
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PhosphoSolutions MAP Kinase Activation Antibody Kit
$459
Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-WAVE1 (N-terminal region) Antibody
$340
Western blot analysis of adult mouse diaphram treated with thapsigargin for 0, 6, 12, or 24 hours to induce cleavage of αII-spectrin from 250 kDa to 136 kDa. The blot was probed with rabbit polyclonal αII-spectrin (a.a. 1171-1176) cleavage-specific antibody at a dilution of 1:1,000 (Image provided by Dr. Leigh Ann Callahan, Department of Internal Medicine, University of Kentucky.)
PhosphoSolutions Anti-αII-Spectrin , cleavage-specific Antibody
$340
Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).
PhosphoSolutions Anti-PTP1B (Tyr-152), Phosphospecific Antibody
$340
Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).
PhosphoSolutions Anti-Paxillin (Tyr-118), Phosphospecific Antibody
$340
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).Formalin fixed, citric acid treated parafin sections of adult Rat striatum. Sections were probed with anti-nNOS (NP2141) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-nNOS (C-terminal region) Antibody
$285
Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Myosin IIA Heavy Chain (Ser-1916), Phosphospecific Antibody
$340
Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).
PhosphoSolutions Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody
$285

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